MATURATION AND HATCHING OF EGGS FROM SILKWORM OVARIES PRESERVED IN LIQUID-NITROGEN
MATURATION AND HATCHING OF EGGS FROM SILKWORM OVARIES PRESERVED IN LIQUID-NITROGEN
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DOI：
10.1016/0022-1910(85)90031-9
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
YAMASHITA, O
中科院分区：
文献类型：
--
作者：
KUSUDA, J;NOGUCHI, T;YAMASHITA, O
用梯度浓度的甘油处理从家蚕（Bombyx mori）的五龄幼虫制备的卵巢成虫盘，以1 ℃的速率冷却。C/min至-35 ℃。在液氮中保存2天或更长时间，然后快速解冻（500 ℃）。C/min）。将冷冻和解冻的卵巢移植到5龄雌性幼虫中，其中20%以上的卵巢根据宿主发育状态发育为具有绒毛膜的成熟卵。通过孤雌激活，成熟卵开始胚胎发生并孵化产生幼虫。约50%的孵化发生在C108 X中发育的卵中。Cambodge宿主中约为10%，在C108 ×宿主中约为10%。Aojuku主机。孵化出的幼虫和正常幼虫一样完成胚后发育。
Ovarian imaginal discs prepared from fifth-instar larvae of the silkworm, Bombyx mori were treated with graded concentrations of glycerol, cooled at a rate of 1.degree. C/min to -35.degree. C and preserved in liquid nitrogen for 2 days or more and then rapidly thawed (500.degree. C/min). The frozen and thawed ovaries were transplanted into fifth-instar female larvae, in which more than 20% of the ovaries developed to produce mature eggs with a chorion according to the state of host development. By parthenogenetic activation, the mature eggs started embryogenesis and hatched to produce larvae. About 50% hatching occurred in the eggs developed in a C 108 .times. Cambodge host, and about 10% in a C 108 .times. Aojuku host. The hatched larvae completed post-embryonic development as did the normal larvae.