Hybridoma autoantibodies to DNA.
Hybridoma autoantibodies to DNA.
复制标题
DNA 杂交瘤自身抗体。
作者：
C. Andrzejewski;B. Stollar;T. Lalor;R. Schwartz
MRL/1小鼠发展为严重形式的系统性红斑狼疮，并自发产生高水平的DNA自身抗体。来自这些动物的脾细胞被用作融合伴侣以制备产生针对DNA的自身抗体的杂交瘤。由于未采用外源免疫，杂交瘤抗体来源于自发产生自身抗体的B细胞群。每种杂交瘤自身抗体与S-DNA、N-DNA或寡核苷酸具有独特的结合特异性。
MRL/1 mice develop a severe form of systemic lupus erythematosus and spontaneously produce high levels of autoantibodies to DNA. Spleen cells from these animals were used as fusion partners to prepare hybridomas that produce autoantibodies to DNA. Since exogenous immunization was not employed, the hybridoma antibodies were derived from populations of B cells that spontaneously produced autoantibodies. Each hybridoma autoantibody had a distinctive binding specificity with S-DNA, N-DNA, or oligonucleotides.