Synergistic antitumor effects of immune cell-viral biotherapy
Synergistic antitumor effects of immune cell-viral biotherapy
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DOI：
10.1126/science.1121411
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发表时间：
2006-03-24
期刊：
影响因子：
56.9
通讯作者：
Contag, CH
中科院分区：
文献类型：
--
作者：
Thorne, SH;Negrin, RS;Contag, CH
靶向生物疗法在癌症治疗方面具有巨大潜力，但其使用受到递送和有效肿瘤靶向限制的限制。我们将免疫效应细胞群[尼古丁诱导的杀伤（CIK）细胞]与溶瘤病毒疗法相结合，以实现免疫缺陷和免疫活性小鼠模型中肿瘤的定向递送和消退。用修饰的牛痘病毒预感染CIK细胞导致长时间的蚀相，病毒保持隐藏，直到与肿瘤相互作用。全身成像显示，这些细胞在释放病毒之前保留了有效地进入和渗透肿瘤的能力。这些结果说明了组合生物治疗剂的协同效应的潜力，有效治疗癌症。
Targeted biological therapies hold tremendous potential for treatment of cancer, yet their use has been limited by constraints on delivery and effective tumor targeting. We combined an immune effector cell population [cytokine-induced killer (CIK) cells] with an oncolytic viral therapy to achieve directed delivery to, and regression of, tumors in both immunodeficient and immunocompetent mouse models. Preinfection of CIK cells with modified vaccinia virus resulted in a prolonged eclipse phase with the virus remaining hidden until interaction with the tumor. Whole-body imaging revealed that the cells retained their ability to traffic to and to infiltrate the tumor effectively before releasing the virus. These results illustrate the potential of combining biotherapeutics for synergistic effects that more. effectively treat cancer.