Differential expression of CRMP1, CRMP2A, CRMP2B, and CRMP5 in axons or dendrites of distinct neurons in the mouse brain
Differential expression of CRMP1, CRMP2A, CRMP2B, and CRMP5 in axons or dendrites of distinct neurons in the mouse brain
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DOI：
10.1002/cne.20465
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发表时间：
2005-05-23
影响因子：
2.5
通讯作者：
Gauchy, C
中科院分区：
文献类型：
--
作者：
Bretin, S;Reibel, S;Gauchy, C
CRMP 1、CRMP 2和CRMP 5已被鉴定为传递信号蛋白3A信号的胞质蛋白，信号蛋白3A信号是传导轴突和树突生长和引导的分子线索之一。它们在脑个体发育过程中高度表达，但是，由于它们在成人中的水平较低，它们在成熟脑中的分布很少被记录。通过使用特异性抗体，我们研究了这些CRMPs在不同成人脑结构和神经细胞培养物中的细胞分布，特别关注剪接变体CRMP 2A和CRMP 2B。在成年小鼠的脑切片中，CRMP 1、CRMP 2B和CRMP 5主要位于特定神经元群体的树突中，例如皮质锥体神经元、海马CA 1锥体细胞或浦肯野小脑细胞。相反，CRMP 2A特异性地与胼胝体的轴突、纹状体的束和海马的苔藓纤维相关。在培养的皮质神经元，CRMP 1，CRMP 2A，CRMP 2B和CRMP 5均匀分布在整个细胞体，轴突，或神经元的树突，而CRMP 2A和CRMP 5是完全不存在的浦肯野小脑细胞在12日龄的动物。相比之下，少突胶质细胞只表达CRMP 2B和CRMP 5的细胞体和过程都在原位在成人大脑和原代培养。总体而言，我们的研究结果表明，CRMP 1，CRMP 2A，CRMP 2B和CRMP 5的特定亚细胞定位取决于细胞类型，神经元区室和发育阶段。这项研究表明，除了它们在轴突生长和指导中的信号传导功能之外，CRMPs还在轴突和树突中的成熟神经元中发挥作用。(c)2005 Wiley-Liss，Inc.
CRMP1, CRMP2, and CRMP5 have been identified as cytosolic proteins relaying semaphorin 3A signalling, one of the molecular cues conducting axon and dendrite growth and guidance. They are highly expressed during brain ontogenesis, but, because of their lower levels in the adult, their distribution in the mature brain is poorly documented. By using specific antibodies, we investigated the cellular distribution of these CRMPs in different adult brain structures and in neural cell cultures with a special focus on the splice variants CRMP2A and CRMP2B. In brain sections of adult mouse, CRMP1, CRMP2B, and CRMP5 were located predominantly in dendrites of specific neuronal populations, such as cortical pyramidal neurons, hippocampal CA1 pyramidal cells, or Purkinje cerebellar cells. On the contrary, CRMP2A was specifically associated with axons of the corpus callosum, bundles of the striatum, and mossy fibers of the hippocampus. In cultures of cortical neurons, CRMP1, CRMP2A, CRMP2B, and CRMP5 were equally distributed throughout cell bodies, axons, or dendrites of neurons, whereas CRMP2A and CRMP5 were completely absent from Purkinje cerebellar cells in 12-day-old animals. By comparison, oligodendrocytes exclusively express CRMP2B and CRMP5 in cell bodies and processes both in situ in the adult brain and in primary cultures. Overall, our results demonstrate specific subcellular localizations of CRMP1, CRMP2A, CRMP2B, and CRMP5 depending on cell types, neuronal compartment, and developmental stage. This study suggests that, beyond their signalling function in axon outgrowth and guidance, CRMPs also play a role in mature neurons both in axons and in dendrites. (c) 2005 Wiley-Liss, Inc.