Quantification of two polyacetylenes in radix ginseng and roots of related Panax species using a gas chromatography-mass spectrometric method
Quantification of two polyacetylenes in radix ginseng and roots of related Panax species using a gas chromatography-mass spectrometric method
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DOI：
10.1021/jf070735o
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发表时间：
2007-10-31
影响因子：
6.1
通讯作者：
Jiang, Zhi-Hong
中科院分区：
文献类型：
--
作者：
Liu, Ji-Hua;Lee, Che-Sum;Jiang, Zhi-Hong
采用毛细管气相色谱-质谱联用（GC-MS）法测定人参中的多炔类化合物人参炔醇和人参醇。选择离子监测（SIM）的检测模式允许灵敏和选择性定量人参中的两种化合物。方法验证表明，GC-MS法的检测限和定量限远低于高效液相色谱（HPLC）-UV法。这表明GC-MS对于分析聚乙炔化合物特别有用，与人参皂苷相比，聚乙炔化合物的丰度相对较低。通过对不同类型人参药材的定量分析，发现林参中人参醇和人参炔醇的含量均高于栽培参。该方法已用于三七和西洋参中人参炔醇和人参醇的定量分析。人参醇与人参炔醇的比值可作为鉴别人参、三七和西洋参的指标。本文首次建立了人参属植物中多炔类化合物的GC-MS定量分析方法。
A sensitive method for quantitating the pharmacologically active polyacetylenes panaxynol and panaxydol in Radix Ginseng was developed using a capillary gas chromatography-mass spectrometric (GC-MS) method. The detection mode of selected ion monitoring (SIM) allowed sensitive and selective quantitation of the two compounds in ginseng. Method validation showed that the GC-MS method has much lower detection and quantitation limits than the high-performance liquid chromatography (HPLC)-UV method. This indicates that GC-MS is particularly useful for the analysis of polyacetylene compounds, which have relatively low abundances compared with ginsenosides in ginseng. Based on the quantitative results of different types of ginseng herbs, it was found that the panaxydol and panaxynol contents were higher in forest ginseng than in cultivated ginseng. This method was further applied to the quantitative analyses of panaxynol and panaxydol in Radix Notoginseng and American ginseng. The ratio of panaxydol to panaxynol can be utilized as a marker for differentiating ginseng, notoginseng, and American ginseng. This study introduces the first GC-MS method for the quantitative analysis of polyacetylenes in herbs of the Panax genus.