Subcellular localizations of AS1 and AS2 suggest their common and distinct roles in plant development.
Subcellular localizations of AS1 and AS2 suggest their common and distinct roles in plant development.
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DOI：
10.1111/j.1744-7909.2008.00693.x
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发表时间：
2008-07
影响因子：
11.4
通讯作者：
Yan Zhu;Ziyu Li;Ben Xu;Hongda Li;Ling-Jian Wang;A. Dong;Hai Huang
中科院分区：
文献类型：
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作者：
Yan Zhu;Ziyu Li;Ben Xu;Hongda Li;Ling-Jian Wang;A. Dong;Hai Huang
在叶器官发生过程中，正常叶原基起始的关键步骤是抑制 1 类 KNOTTED1 样同源框 (KNOX) 基因。叶原基形成后，它们必须建立正常叶形态发生的极性。最近的研究已经鉴定出许多参与 1 类 KNOX 基因抑制和/或叶极性建立的基因。 ASTMMETRIC LEAVES1 和 2（AS1 和 AS2）是其中两个基因，它们对于这两个过程都至关重要。作为了解 AS1-AS2 作用分子遗传基础的第一步，我们通过将这两种蛋白与黄色/青色荧光蛋白 (YFP/CFP) 融合，确定了烟草 BY2 细胞和拟南芥植物中这两种蛋白的亚细胞定位。我们的数据表明，AS1 和 AS2 单独主要分别位于核仁和核质中。 AS1 和 AS2 蛋白在同一间期细胞中的存在证明了它们在核仁和核质中的共定位。此外，AS1单独能够与中期细胞中的浓缩染色体结合。我们的数据表明 AS1、AS2 和 AS1-AS2 蛋白复合物可能具有不同的功能，这些功能都是正常植物发育所必需的。
During leaf organogenesis, a critical step for normal leaf primordium initiation is the repression of the class 1 KNOTTED1-like homeobox (KNOX) genes. After leaf primordia are formed, they must establish polarity for normal leaf morphogenesis. Recent studies have led to the identification of a number of genes that participate in the class 1 KNOX gene repression and/or the leaf polarity establishment. ASTMMETRIC LEAVES1 and 2 (AS1 and AS2) are two of these genes, which are critical for both of these two processes. As a first step towards understanding the molecular genetic basis of the AS1-AS2 action, we determined the subcellular localizations of the two proteins in both tobacco BY2 cells and Arabidopsis plants, by fusing them to yellow/cyan fluorescent protein (YFP/CFP). Our data showed that AS1 and AS2 alone were predominantly localized in the nucleolus and the nucleoplasm, respectively. The presence of both AS1 and AS2 proteins in the same interphase cell demonstrated their co-localization in both nucleolus and nucleoplasm. In addition, AS1 alone was able to associate with the condensed chromosome in the metaphase cell. Our data suggest that AS1, AS2 and the AS1-AS2 protein complex may have distinct functions, which are all required for normal plant development.