Dependence-induced alcohol drinking by alcohol-preferring (P) rats and outbred Wistar rats
Dependence-induced alcohol drinking by alcohol-preferring (P) rats and outbred Wistar rats
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DOI：
10.1111/j.1530-0277.2008.00678.x
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发表时间：
2008-09-01
影响因子：
3.2
通讯作者：
Koob, G. F.
中科院分区：
文献类型：
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作者：
Gilpin, N. W.;Richardson, H. N.;Koob, G. F.
背景：多年来，慢性间歇性酒精蒸气暴露和选择性育种程序已分别用于模拟酒精依赖的特定方面。本研究的目的是将这两种方法结合起来，通过将酒精偏好(P)大鼠暴露在慢性间歇性酒精蒸气中较长时间，然后在蒸汽暴露结束后的多个时间点与一组近交系Wistar大鼠平行测试它们对操作性酒精的反应。方法：将P大鼠（n = 20）和Wistar大鼠（n = 18）分别训练成在操作状态下对10% （w/v）乙醇产生反应，然后根据摄入水平分为各组。然后将动物暴露于慢性间歇性酒精蒸气（14小时开/10小时关）或空气中8周。然后对大鼠在酒精戒断（6小时）和长期戒断（1至15天）期间的不同条件和多个时间点进行操作性酒精反应测试。结果：慢性酒精蒸气暴露在P大鼠和Wistar大鼠急性戒断和长期戒断期间产生类似的操作性酒精反应增加。结论：这些结果说明了遗传选择对高度酒精偏好和酒精依赖行为的单独和联合影响。此外，这些结果证实了过去的发现，即依赖的大鼠比不依赖的对照组消耗更多的酒精，并在长时间的酒精蒸汽暴露后戒酒。
Background: Chronic intermittent alcohol vapor exposure and selective breeding procedures have been used separately for many years to model specific aspects of alcohol dependence. The purpose of the present investigation was to combine these 2 approaches by exposing alcohol-preferring (P) rats to chronic intermittent alcohol vapor for extended periods of time and then testing them for operant alcohol responding in parallel with a group of outbred Wistar rats at multiple time points following the termination of vapor exposure.Methods: P rats (n = 20) and Wistar rats (n = 18) were trained to respond for 10% (w/v) ethanol in an operant situation, then divided into groups matched for intake levels. Animals were then exposed to chronic intermittent alcohol vapor (14 hours ON/10 hours OFF) or air for 8 weeks. Rats were then tested for operant alcohol responding under various conditions and at multiple time points during alcohol withdrawal (6 hours) and protracted abstinence (1 to 15 days).Results: Chronic alcohol vapor exposure produced similar increases in operant alcohol responding in P rats and Wistar rats during acute withdrawal and protracted abstinence.Conclusions: These results illustrate the separate and combined effects of genetic selection for high alcohol preference and dependence on alcohol drinking behavior. Furthermore, these results confirm past findings that dependent rats consume more alcohol than nondependent controls well into abstinence following extended periods of alcohol vapor exposure.