Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity
Inhibitory Effect of Eriodictyol on IgE/Ag-Induced Type I Hypersensitivity
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DOI：
10.1271/bbb.110952
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
J. Yoo;Ji-Hee Kim;Sae-Jin Park;Yeo-Jin Kang;Tack-Joong Kim
中科院分区：
文献类型：
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作者：
J. Yoo;Ji-Hee Kim;Sae-Jin Park;Yeo-Jin Kang;Tack-Joong Kim
肥大细胞是通过释放组胺参与过敏反应的主要效应细胞。我们研究了圣草酚，来自画枫树和圣诞老人，对肥大细胞脱颗粒和过敏反应的动物模型的影响。我们还研究了其对参与钙依赖性脱粒的神经酰胺激酶（CERK）表达的影响，以及对多种细胞因子激活神经酰胺的影响。圣草酚抑制释放β-氨基己糖苷酶，脱粒的标志物，和白细胞介素（IL）-4 mRNA的表达。它抑制CERK mRNA的表达，降低抗原刺激的肥大细胞中神经酰胺的浓度，并以剂量依赖性方式抑制小鼠被动皮肤过敏反应（PCA）。这些结果表明，圣草酚可以通过抑制神经酰胺激酶抑制肥大细胞脱粒，它可能作为一种潜在的抗过敏剂。
Mast cells are the principal effector cells involved in the allergic response, through the release of histamine. We investigated the effect of eriodictyol, derived from the painted maple and yerba santa, on mast cell degranulation and on an allergic response in an animal model. We also investigated its effect on the expression of the ceramide kinase (CERK) involved in calcium-dependent degranulation, and on ceramide activation by multiple cytokines. Eriodictyol suppressed the release of beta-hexosaminidase, a marker of degranulation, and the expression of interleukin (IL)-4 mRNA. It inhibited the expression of CERK mRNA, reduced the ceramide concentration in antigen-stimulated mast cells, and suppressed the passive cutaneous anaphylaxis (PCA) reaction in mice in a dose-dependent manner. These results suggest that eriodictyol can inhibit mast cell degranulation through inhibition of ceramide kinase, and that it might potentially serve as an anti-allergic agent.