Exploring the antioxidant property of bioflavonoid quercetin in preventing DNA glycation: A calorimetric and spectroscopic study
Exploring the antioxidant property of bioflavonoid quercetin in preventing DNA glycation: A calorimetric and spectroscopic study
复制标题
DOI：
10.1016/j.bbrc.2005.11.019
复制
发表时间：
2006-01-06
影响因子：
3.1
通讯作者：
Swenson, J
中科院分区：
文献类型：
--
作者：
Sengupta, B;Uematsu, T;Swenson, J
还原糖，如葡萄糖、果糖等及其磷酸盐衍生物，非酶糖化生物大分子(如蛋白质、DNA和脂类)，与自由基的产生有关。利用差示扫描量热法(DSC)、紫外可见吸收光谱(UV/Vis)和光子相关光谱(PCS)对正常和糖化的人胎盘DNA进行了一项新的研究，并探讨了天然存在的多羟基黄酮(3，3‘，4’，5，7-五羟基黄酮)在防止糖基化方面的抗氧化性能。DNA在糖存在下吸收强度的降低清楚地表明在双链DNA分子中碱基对的两个碱基之间存在糖分子。正常和糖化DNA的PCS弛豫谱有明显的变化。糖化时胎盘DNA的融化温度降低，表明双链糖化DNA的结构稳定性降低。我们的DSC和PCS数据首次表明，添加槲皮素可以在很大程度上防止糖化DNA结构性质的戏剧性变化。这项研究对正常和糖化的DNA分子的结构、功能和动力学提供了有价值的见解，为自由基介导的疾病的表现以及使用具有治疗活性的天然黄酮类Qercetin预防疾病提供了基础。(C)2005 Elsevier Inc.保留所有权利。
Reducing sugars for example glucose, fructose, etc., and their phosphate derivatives non-enzymatically glycate biological macromolecules (e.g., proteins, DNA and lipids) and is related to the production of free radicals. Here we present a novel study, using differential scanning calorimetry (DSC) along with UV/Vis absorption and photon correlation spectroscopy (PCS), on normal and glycated human placenta DNA and have explored the antioxidant property of the naturally occurring polyhydroxy flavone quercetin (3,3',4',5,7-penta-hydroxyflavone) in preventing the glycation. The decrease in the absorption intensity of DNA in presence of sugars clearly indicates the existence of sugar molecules between the two bases of a base pair in the duplex DNA Molecule. Variations were perceptible in the PCS relaxation profiles of normal and glycated DNA. The melting temperature of placenta DNA was decreased when glycated suggesting a decrease in the structural stability of the double-stranded glycated DNA. Our DSC and PCS data showed, for the first time, that the dramatic changes in the Structural properties of glycated DNA can be prevented to a significant extent by adding quercetin. This study provides Valuable insights regarding the structure, function, and dynamics of normal and glycated DNA molecules, underlying the manifestation of free radical mediated diseases, and their prevention using therapeutically active naturally occurring flavonoid quercetin. (c) 2005 Elsevier Inc. All rights reserved.