RecQL1 DNA repair helicase: A potential tumor marker and therapeutic target against hepatocellular carcinoma
RecQL1 DNA repair helicase: A potential tumor marker and therapeutic target against hepatocellular carcinoma
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DOI：
10.3892/ijmm_00000375
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发表时间：
2010-04-01
影响因子：
5.4
通讯作者：
Furuichi, Yasuhiro
中科院分区：
文献类型：
--
作者：
Futami, Kazunobu;Ogasawara, Sachiko;Furuichi, Yasuhiro
RecQL 1在人类RecQ DNA解旋酶家族中参与细胞周期复制中的DNA修复和重组途径对人类肝细胞癌（HCC）组织的免疫组化分析显示RecQL 1表达与HCC的组织学分级和MIB-1指数密切相关。在单纯性肝癌中，诱导门静脉浸润结节性生长的肝癌中，其表达高于未诱导门静脉浸润结节性生长的肝癌。这些组织学数据揭示了RccQL 1作为预测肝脏恶性和进展的生物标志物的潜力。各种HCC细胞也产生高表达谱。包括当RccQL 1表达被siRNA沉默时，通过LIS建立的HCC细胞系。大多数HCC细胞死于有丝分裂灾难在移植人HCC的肝癌小鼠原位异种移植模型中。RecQL 1-siRNA与阳离子脂质体混合后显示出较强的抑制肿瘤生长的作用。证实RecQL 1是抗肝癌的优良分子剂，并提示用肝倾向脂质体配制的RecQL 1-siRNA具有作为抗肝癌的治疗药物的优良潜力。
RecQL1 in the human RecQ DNA helicase family participates in DNA repair and recombination pathways in cell cycle replication Immunohistochemical analysis Of human hepatocellular carcinoma (HCC) tissues showed that RecQL1 expression is strongly correlated with histological grade and MIB-1 indices of HCC. and that the expression was greater in simple HCCs inducing extranodular growth of portal vein invasion than in HCCs not inducing extranodular growth of Portal well invasion. These histological data reveal the potential of RccQL1 as a biological marker predicting the malignancy and progression of liver Cancel. High expression profiles were also produced by various HCC cells. including, HCC cell lines established by LIS When RccQL1 expression was silenced by siRNA in vitro. most HCC cells died of mitotic catastrophe In a mouse orthotopic xenograft model of liver cancer with transplanted human HCC. RecQL1-siRNA mixed with cationic liposomes exhibited I strong anticancer effect that prevented the growth of the cancer RecQL1-siRNA inhibited the growth Of HCC in file mouse liver. confirming that RecQL1 is an excellent molecular agent against liver cancer and suggests that RecQL1-siRNA formulated With liver-prone liposomes has excellent potential as a therapeutic drug against liver cancers.