Natural killer cell reprogramming with chimeric immune receptors.
Natural killer cell reprogramming with chimeric immune receptors.
复制标题
用嵌合免疫受体对自然杀伤细胞进行重编程。
DOI：
10.1007/978-1-62703-260-5_13
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Campana,Dario
中科院分区：
文献类型：
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作者：
Shimasaki,Noriko;Campana,Dario
自然杀伤（NK）细胞正在成为癌症细胞治疗的新工具。然而，一些癌症亚型对NK细胞的细胞毒性具有相对抗性。抗CD19嵌合信号受体的表达可以增强NK细胞对CD19+白血病和淋巴瘤细胞的反应性。在这里，我们描述了一种方法，以加强这种受体在人类NK细胞的表达依赖于电穿孔的mRNA，并比较它的逆转录病毒转导的cDNA。这些方法适用于用对癌细胞上表达的其他抗原特异性的嵌合受体以及用可调节NK细胞功能的分子重编程NK细胞。
Natural killer (NK) cells are emerging as a new tool for cell therapy of cancer. However, some cancer subtypes are relatively resistant to NK cell cytotoxicity. Expression of anti-CD19 chimeric signaling receptors can enhance NK-cell reactivity against CD19+ leukemia and lymphoma cells. Here we describe a method to enforce expression of such receptors in human NK cells relying on electroporation of mRNA and compare it to retroviral transduction of cDNA. These methods are applicable to the reprogramming of NK cells with chimeric receptors specific for other antigens expressed on cancer cells as well as with molecules that can modulate NK cell function.