alpha-lipoic acid corrects late-phase supernormal retinal oxygenation response in experimental diabetic retinopathy.
alpha-lipoic acid corrects late-phase supernormal retinal oxygenation response in experimental diabetic retinopathy.
复制标题
α-硫辛酸可纠正实验性糖尿病视网膜病变的晚期超正常视网膜氧合反应。
DOI：
10.1167/iovs.06-0464
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发表时间：
2006
影响因子：
4.4
通讯作者：
Berkowitz,BruceA
中科院分区：
文献类型：
--
作者：
Roberts,Robin;Luan,Hongmei;Berkowitz,BruceA
purpose. To test the hypothesis that preventative α-lipoic acid (LPA) treatment corrects an abnormal retinal oxygenation response in experimental diabetic retinopathy.methods. Retinal oxygenation (ΔP o 2) was measured by MRI before (room air [ra]) and during a 4-minute carbogen inhalation challenge in five groups: control Sprague-Dawley (SD) and Lewis (LEW) rats, 3-to 4-month diabetic SD and LEW rats, and 4-month diabetic LEW rats preventatively treated with a chow LPA admix (400 mg/kg per chow). Comparisons were made between the initial 2 minutes of oxygenation change (measured using ra and first carbogen periods [t 1− ra]) and the next 2-minute change (assessed with first and second carbogen periods [t 2− t 1]) for superior and inferior hemiretinal ΔP o 2.results. In control SD rats, ΔP o 2 (t 1− ra)> ΔP o 2 (t 2− t 1)(P< 0.05) was found panretinally. In diabetic SD rats, the superior, but not the inferior, hemiretina had subnormal ΔP o 2 (t 1− ra)(P< 0.05) and supernormal ΔP o 2 (t 2− t 1)(P< 0.05). In control LEW rats, ΔP o 2 (t 1− ra) and ΔP o 2 (t 2− t 1) were not significantly different (P> 0.05). Also, control and diabetic LEW rat panretinal ΔP o 2 (t 1− ra) were lower (P< 0.05) than in the respective SD groups. In diabetic LEW rats, a supernormal (P< 0.05) panretinal ΔP o 2 (t 2− t 1) was found that could be corrected with preventative LPA treatment.conclusions. These data support the hypothesis and suggest that the influence of strain differences on the interpretation of retinovascular ΔP o 2 as a surrogate of drug treatment efficacy for diabetic retinopathy may be minimized by measuring a late-phase supernormal response. The LPA data raise the possibility that oxidative stress contributes to diabetes-induced supernormal ΔP o 2.
