Genotypes of invasive pneumococcal isolates recently recovered from Italian patients
Genotypes of invasive pneumococcal isolates recently recovered from Italian patients
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DOI：
10.1128/jcm.40.10.3660-3665.2002
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发表时间：
2002-10-01
影响因子：
9.4
通讯作者：
Beall, B
中科院分区：
文献类型：
--
作者：
Dicuonzo, G;Gherardi, G;Beall, B
我们检查了意大利选定地区最近分离的73株侵袭性肺炎球菌的基因变异性。共发现33种基因组大限制性内切酶类型，其中3种代表多种血清型。Pbp2b、pbp2x和PSPA的限制性片段模式在大多数具有相同大限制性内切酶类型的分离物中是保守的。在22个青霉素不敏感肺炎链球菌(PNSP)分离株中发现的9个大限制性内切酶类型中，有7个分离株的等位基因图谱与多位点序列分型数据库(www.mlst.net)的图谱显示5-7个等位基因匹配；然而，7个图谱中的3个代表以前与这些克隆簇无关的血清型。两种PNSP宏限制性类型代表了具有独特等位基因图谱的新克隆。在51株对青霉素敏感的肺炎链球菌(PSSP)中，从25种大限制性类型中的3种分离株获得的等位基因图谱与先前描述的图谱密切相关。其中1个PSSP分离物为与多抗克隆France(9V)-3相关的新型24F分离物。这项工作报告了新的PNSP毒株和新的血清型-克隆关联。
We examined 73 recent invasive pneumococcal isolates within selected areas of Italy for genotypic variability. Thirty-three genomic macrorestriction types were found, three of which represented multiple serotypes. Restriction fragment patterns of pbp2b,pbp2x, and pspA were conserved within the majority of isolates that shared macrorestriction types. Of the nine macrorestriction types found among the 22 penicillin-non susceptible Streptococus pneumoniae (PNSP) isolates, seven comprised isolates with allelic profiles showing five to seven allelic matches to profiles in the multilocus sequence typing database (www.mlst.net); however, three of the seven profiles represented serotypes not previously associated with these clonal clusters. Two PNSP macrorestriction types represented new clones with unique allelic profiles. Allelic profiles obtained from isolates of 3 of the 25 macrorestriction types found among the 51 penicillin-susceptible S. pneumoniae (PSSP) isolates were closely related to previously described profiles. One PSSP isolate was a novel type 24F isolate related to the multiresistant clone France(9V)-3. This work reports new PNSP strains and new serotype-clone associations.