RECOMBINATION IN ADAPTIVE MUTATION
RECOMBINATION IN ADAPTIVE MUTATION
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DOI：
10.1126/science.8146657
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发表时间：
1994-04-08
期刊：
影响因子：
56.9
通讯作者：
ROSENBERG, SM
中科院分区：
文献类型：
--
作者：
HARRIS, RS;LONGERICH, S;ROSENBERG, SM
大肠杆菌适应性突变的遗传要求与RecBCD途径中同源重组的遗传要求相似。重组缺陷型recA和recB无效突变株在适应性回复中有缺陷。超重组基因recD菌株是超突变的，其超突变取决于功能性recA和recB基因。不需要次级重组系统的基因。这些结果表明，发生适应性突变的分子机制包括重组。生长中的细胞自发突变没有这种关联。
The genetic requirements for adaptive mutation in Escherichia coli parallel those for homologous recombination in the RecBCD pathway. Recombination-deficient recA and recB null mutant strains are deficient in adaptive reversion. A hyper-recombinagenic recD strain is hypermutable, and its hypermutation depends on functional recA and recB genes. Genes of subsidiary recombination systems are not required. These results indicate that the molecular mechanism by which adaptive mutation occurs includes recombination. No such association is seen for spontaneous mutation in growing cells.