Promoter‐upstream activator sequences are required for expression of the xylS gene and upper‐pathway operon on the Pseudomonas TOL plasmid
Promoter‐upstream activator sequences are required for expression of the xylS gene and upper‐pathway operon on the Pseudomonas TOL plasmid
复制标题
假单胞菌 TOL 质粒上 xylS 基因和上游途径操纵子的表达需要启动子上游激活序列
作者：
A. Holtel;M. Abril;S. Marqués;K. Timmis;J. Ramos
Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps) and the upperpathway operon promoter (Pu) is dependent on the positive regulator protein XylR activated by an effector molecule such as 3‐cholorotoluene, and on RpoN, an RNA polymerase sigma factor. Mutational analysis of the Ps and Pu promoters showed that upstream activator sequences located between ‐110 and ‐218bp upstream of the main transcription initiation point are required for regulated expression from these promoters. A search for homologous nucleotide sequences in the ‐110to ‐218bp region in Pu and Ps revealed conserved sequences that may act as putative recognition sequences for the XylR protein. Ps and Pu exhibit another well‐conserved region at around 50 bp, which is homologous to corresponding sites in other RpoN‐dependent promoters and may constitute a binding site for integration host factor (IHF).
DOI：
10.1073/pnas.82.22.7525
发表时间：
1985-01-01
影响因子：
11.1
作者：
HIRSCHMAN, J;WONG, PK;KUSTU, S
通讯作者：
KUSTU, S
探究大肠杆菌glnALG上游体内激活机制。
DOI：
10.1073/pnas.85.23.8934
发表时间：
1988
影响因子：
11.1
作者：
Sasse-Dwight,S;Gralla,JD
通讯作者：
Gralla,JD