2 TYPES OF CALCIUM RESPONSE LIMITED TO SINGLE SPINES IN CEREBELLAR PURKINJE-CELLS
2 TYPES OF CALCIUM RESPONSE LIMITED TO SINGLE SPINES IN CEREBELLAR PURKINJE-CELLS
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DOI：
10.1073/pnas.92.18.8279
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发表时间：
1995-08-29
影响因子：
11.1
通讯作者：
LLINAS, R
中科院分区：
文献类型：
--
作者：
DENK, W;SUGIMORI, M;LLINAS, R
在理解神经元功能中，最重要的是明确确定神经元整合的最小单位。最近有人提出，一个完整的树突状小枝，包括几十个刺，作为基本单位在浦肯野细胞的树突钙动力学。相比之下，我们证明，最小的这样的单位是单一的脊椎。结果表明，通过双光子激发荧光激光扫描显微镜，个别的棘能够独立的钙激活。此外，两个不同的脊柱人口的区别，其相反的反应膜超极化。事实上，在棘的亚群中，钙离子也可以通过电压门控通道以外的途径进入。这些发现挑战了一个独特的平行纤维激活模式的假设，并促使重新评估归因于单个神经元的功能复杂性水平。
Of fundamental importance in understanding neuronal function is the unambiguous determination of the smallest unit of neuronal integration. It was recently suggested that a whole dendritic branchlet, including tens of spines, acts as the fundamental unit in terms of dendritic calcium dynamics in Purkinje cells. By contrast, we demonstrate that the smallest such unit is the single spine. The results show, by two-photon excited fluorescence laser scanning microscopy, that individual spines are capable of independent calcium activation. Moreover, two distinct spine populations were distinguished by their opposite response to membrane hyperpolarization. Indeed, in a subpopulation of spines calcium entry can also occur through a pathway other than voltage-gated channels. These findings challenge the assumption of a unique parallel fiber activation mode and prompt a reevaluation of the level of functional complexity ascribed to single neurons.