Component identification of electron transport chains in curdlan-producing Agrobacterium sp. ATCC 31749 and its genome-specific prediction using comparative genome and phylogenetic trees analysis
Component identification of electron transport chains in curdlan-producing Agrobacterium sp. ATCC 31749 and its genome-specific prediction using comparative genome and phylogenetic trees analysis
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DOI：
10.1007/s10295-010-0810-x
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发表时间：
2011-06
影响因子：
3.4
通讯作者：
Hongtao Zhang;J. Setubal;X. Zhan;Z. Zheng;Lijun Yu;Jianrong Wu;Dingqiang Chen
中科院分区：
文献类型：
--
作者：
Hongtao Zhang;J. Setubal;X. Zhan;Z. Zheng;Lijun Yu;Jianrong Wu;Dingqiang Chen
Agrobacteriumsp. ATCC 31749 (formerly namedAlcaligenes faecalisvar.myxogenes) is a non-pathogenic aerobic soil bacterium used in large scale biotechnological production of curdlan. However, little is known about its genomic information. DNA partial sequence of electron transport chains (ETCs) protein genes were obtained in order to understand the components of ETC and genomic-specificity inAgrobacteriumsp. ATCC 31749. Degenerate primers were designed according to ETC conserved sequences in other reported species. DNA partial sequences of ETC genes inAgrobacteriumsp. ATCC 31749 were cloned by the PCR method using degenerate primers. Based on comparative genomic analysis, nine electron transport elements were ascertained, including NADH ubiquinone oxidoreductase, succinate dehydrogenase complex II, complex III, cytochromec, ubiquinone biosynthesis protein ubiB, cytochromedterminal oxidase, cytochromeboterminal oxidase, cytochromecbb3-type terminal oxidase and cytochromecaa3-type terminal oxidase. Similarity and phylogenetic analyses of these genes revealed that among fully sequencedAgrobacteriumspecies,Agrobacteriumsp. ATCC 31749 is closest toAgrobacterium tumefaciensC58. Based on these results a comprehensive ETC model forAgrobacteriumsp. ATCC 31749 is proposed.
