Oxidative stress and insulin requirements in patients with recent-onset type 1 diabetes
Oxidative stress and insulin requirements in patients with recent-onset type 1 diabetes
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DOI：
10.1210/jc.2002-021525
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发表时间：
2003-04-01
影响因子：
5.8
通讯作者：
Hobbs, G
中科院分区：
文献类型：
--
作者：
Hoeldtke, RD;Bryner, KD;Hobbs, G
本研究的目的是分析1型糖尿病早期氧化应激的生化指标，并评估其与胰岛素需求的关系。37名患者参加了一项为期3年的纵向研究，研究氧化应激对这种疾病早期自然史的影响。我们测量了血浆亚硝酸盐和硝酸盐（统称为NOx）、硝基酪氨酸和8-异-前列腺素f - 2α（8-异- pgf (2α)）。第1、2、3次评价时，对照组血浆NOx为34.0 +/- 4.9 mumol/l，糖尿病组分别为52.4 +/- 5.1、50.0 +/- 5.1、49.0 +/- 5.2 mumol/l （P < 0.01）。对照组硝基酪氨酸为13.3 +/- 2.0 mu mol/l，糖尿病组为26.8 +/- 4.4、26.1 +/- 4.3、32.7 +/- 4.3 mu mol/l （P < 0.01）。8-Iso-PGF（2alpha）在控制不良的患者中高于控制良好的患者。第1次、第2次、第3次评价NOx与胰岛素剂量相关（P < 0.05）。8-Iso-PGF（2 α）与胰岛素剂量在第一次评估（P < 0.01）和第三次评估（P < 0.0025）相关。氧化应激的系统性测量与早期1型糖尿病的胰岛素需求相关。这些结果表明，氧化应激正在胰腺中发生，并损害β细胞。
The purpose of this study was to analyze biochemical measures of oxidative stress and assess their relationship to insulin requirements early in type 1 diabetes. Thirty-seven patients enrolled in a 3-yr longitudinal study of the effects of oxidative stress on the early natural history of this disorder. We measured plasma nitrite and nitrate (collectively NOx), nitrotyrosine, and 8-iso-prostaglandin F-2alpha (8-iso-PGF(2alpha)). Plasma NOx was 34.0 +/- 4.9 mumol/liter in the control subjects and 52.4 +/- 5.1, 50.0 +/- 5.1, and 49.0 +/- 5.2 mumol/liter in the diabetic patients at the first, second, and third evaluations, respectively (P < 0.01). Nitrotyrosine was 13.3 +/- 2.0 mu mol/liter in controls and 26.8 +/- 4.4, 26.1 +/- 4.3, and 32.7 +/- 4.3 mu mol/liter in the diabetic patients (P < 0.01). 8-Iso-PGF(2alpha) was higher in the poorly controlled than in the well controlled patients. NOx correlated with insulin dose at the first (P < 0.05), second (P < 0.025), and third (P < 0.05) evaluations. 8-Iso-PGF(2 alpha) correlated with insulin dose at the first (P < 0.01) and third (P < 0.0025) evaluations.Systemic measures of oxidative stress correlate with insulin requirements in early type 1 diabetes. These results suggest that oxidative stress is taking place in the pancreas and damaging the beta-cell.