Hydroxyapatite formed on/in agarose gel induces activation of blood coagulation and platelets aggregation
Hydroxyapatite formed on/in agarose gel induces activation of blood coagulation and platelets aggregation
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DOI：
10.1002/jbm.b.30684
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发表时间：
2007-05-01
影响因子：
3.4
通讯作者：
Maruyama, Ikuro
中科院分区：
文献类型：
--
作者：
Arimura, Shin-ichiro;Kawahara, Ko-ichi;Maruyama, Ikuro
我们之前报道过交替浸泡法制备的琼脂糖凝胶（HA/琼脂糖）上形成的羟基磷灰石（HA）是一种具有骨导电性和止血作用的骨填充材料。这个过程可以让我们制造出在有机聚合物水凝胶基质上形成的类骨磷灰石。我们研究了HA/琼脂糖诱导的止血机制，发现HA/琼脂糖而不是琼脂糖或HA粉能显著缩短活化的部分凝血活素时间（APTT）。虽然HA/琼脂糖没有明显的血小板聚集，但它明显增强了二磷酸腺苷（ADP）诱导的血小板聚集。此外，Western blot分析显示玻璃体粘连蛋白在HA/琼脂糖上的选择性吸附。我们还观察到HA粉和HA/琼脂糖的XRD谱图有明显的差异。透明质酸粉末的结晶度比透明质酸/琼脂糖高得多。在HA粉末中观察到50 ~ 100 nm的管状聚集体，在HA/琼脂糖中观察到100 ~ 200 nm的颗粒。因此，在透明质酸/琼脂糖表面结构上的100-200缸低结晶颗粒可能在止血中起重要作用。我们的研究结果证明了透明质酸/琼脂糖在止血机制中的重要作用，并提示了透明质酸/琼脂糖作为植骨材料的潜在作用。(c) 2006 Wiley期刊公司
We reported earlier that hydroxyapatite (HA) formed on/in agarose gels (HA/ agarose) produced by alternate soaking process is a bone-filling material possessing osteoconductive and hemostatic effects. This process could allow us to make bone-like apatite that was formed on/in organic polymer hydrogel matrices. Here, we investigated the mechanism of hemostasis induced by HA/agarose and found that HA/agarose, but not agarose or HA powder, significantly shortened activated partial thromboplastin time (APTT). While HA/agarose did not show significant platelet aggregation, it markedly enhanced adenosine diphosphate (ADP)-induced platelet aggregation. Moreover, Western blot analysis revealed selective adsorption of vitronectin onto HA/agarose. We also observed marked differences between HA powder and HA/agarose in their XRD patterns. The crystallinity of HA powder was much higher compared to that of HA/agarose. Furthermore, 50-100 nm of tube-form aggregations was observed in HA powder on the other hand 100-200 nm of particles was observed in HA/agarose by SEM observation. Thus 100-200 urn of low crystallized particles on the surface structure of HA/agarose may play an important role in hemostasis. Our results demonstrated a crucial role of HA/agarose in the mechanism of hemostasis and suggested a potential role for HA/agarose as a bone-grafting material. (c) 2006 Wiley Periodicals, Inc.