Accelerated bone formation on photo-induced hydrophilic titanium implants: an experimental study in the dog mandible
Accelerated bone formation on photo-induced hydrophilic titanium implants: an experimental study in the dog mandible
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DOI：
10.1111/j.1600-0501.2011.02401.x
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发表时间：
2013-08-01
影响因子：
4.3
通讯作者：
Sawase, Takashi
中科院分区：
文献类型：
--
作者：
Hirakawa, Yuko;Jimbo, Ryo;Sawase, Takashi
目的：本研究的目的是研究光诱导亲水性二氧化钛（TiO 2）对血清纤维连接蛋白（sFN）附着的影响，并进一步评估狗下颌骨中的初始骨整合反应。材料和方法：为了施加纳米TiO 2膜，对钛盘和植入物采用等离子体源离子注入（PSII）方法，随后进行退火，然后对实验组用UV-A照射24 h。为对照组制备非沉积钛盘和植入物。使用干涉仪和接触角分析仪进行表面表征。使用荧光发射分析评价sFN的附着。此后，将两组种植体放置在6只比格犬的下颌骨中。结果：实验组在UV-A照射下表现出较强的亲水性，sFN的粘附能力明显增强。结论：血浆纤维连接蛋白与PSII联合应用制备亲水性钛表面可促进早期骨整合。
Objectives: The purpose of this study was to investigate the effect of photo-induced hydrophilic titanium dioxide (TiO2) on serum fibronectin (sFN) attachment, and further to evaluate initial osseointegration responses in the dog mandibles.Materials and methods: To apply the anatase TiO2 film, plasma source ion implantation (PSII) method followed by annealing was employed for the titanium disks and implants, which were then illuminated with UV-A for 24h for the experimental groups. Non-deposited titanium disks and implants were prepared for the control group. Surface characterization was performed using the interferometer and contact angle analyzer. The attachments of sFN were evaluated using fluorescence emission analysis. Thereafter both groups of implants were placed in the mandible of six beagle dogs. Bone response was investigated with histological and histomorphometrical analyses after periods of 2 and 4weeks.Results: The experimental groups exhibited strong hydrophilicity under UV-A illumination and showed significant improvement in sFN attachment. And further, the experimental implants enhanced the bone formation with the bone-to-implant contact of 42.7% after 2weeks of healing (control: 28.4%).Conclusions: The combined applications of plasma fibronectin and PSII to produce hydrophilic titanium surfaces could accelerate early osseointegration.