CARDITIS IN LYME-DISEASE SUSCEPTIBLE AND RESISTANT STRAINS OF LABORATORY MICE INFECTED WITH BORRELIA-BURGDORFERI
CARDITIS IN LYME-DISEASE SUSCEPTIBLE AND RESISTANT STRAINS OF LABORATORY MICE INFECTED WITH BORRELIA-BURGDORFERI
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DOI：
10.4269/ajtmh.1992.47.249
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发表时间：
1992-08-01
影响因子：
3.3
通讯作者：
BECK, DS
中科院分区：
文献类型：
--
作者：
ARMSTRONG, AL;BARTHOLD, SW;BECK, DS
在皮内接种伯氏疏螺旋体菌株N40后第3、6、10、15、30、60和90天，在4周龄易感C3 H/He（C3 H）和耐药C57 B1/6（B6）小鼠中评价心脏莱姆病的临床和病理演变。采用培养、DNA聚合酶链反应、原位核酸杂交、免疫组化和银染色等方法检测螺旋体。第6天，在4只C3 H小鼠中的2只中通过培养首次检测到螺旋体。两种基因型的所有小鼠的心脏在第10天培养物呈阳性，并且感染持续至第90天。螺旋体对心脏基底部的结缔组织，特别是主动脉周围、上心室和心房的心外膜、心肌肥厚和内膜有偏爱。在第10天首次检测到心脏炎，在第15天达到最严重程度，然后消退，除了C3 H小鼠中持续至第90天和B6小鼠中持续至第60天的主动脉周围淋巴浆细胞浸润。C3 H小鼠比B6小鼠发生更严重的疾病，这与C3 H小鼠中螺旋体的出现更早、数量更多和清除更晚有关。感染和对照小鼠的心电图显示心动过缓和心动过速在许多C3 H小鼠，但在极少数B6小鼠。血清肌酐磷酸激酶水平在任何时间间隔均未升高。
The clinical and pathologic evolution of cardiac Lyme disease was evaluated in four-week-old susceptible C3H/He (C3H) and resistant C57B1/6 (B6) mice on days 3, 6, 10, 15, 30, 60, and 90 after intradermal inoculation with Borrelia burgdorferi strain N40. Culture, DNA polymerase chain reaction, in situ nucleic acid hybridization, immunoperoxidase histochemical analysis, and silver stain were used to detect spirochetes. Spirochetes were first detected by culture on day 6 in two of four C3H mice. The hearts of all mice of both genotypes were culture positive by day 10 and infection persisted through day 90. The spirochetes had a predilection for connective tissue in the heart base, especially around the aorta, epicardium of the upper ventricles and atria, myocardial interstitium, and endocardium. Carditis was first detectable on day 10, reaching a maximum severity on day 15, then resolved, except for persistence of periaortic lymphoplasmacytic infiltrates through day 90 in C3H mice and through day 60 in B6 mice. The C3H mice developed more severe disease than the B6 mice, and this was associated with the earlier appearance, greater numbers, and later clearance of spirochetes in C3H mice. Electrocardiographs of infected and control mice revealed bradycardia and tachycardia in many C3H mice, but in very few B6 mice. Serum creatinine phosphokinase levels did not become elevated at any interval.