Avian thymic hormone and chicken (muscle) parvalbumin are distinct proteins: isolation of a muscle parvalbumin cDNA fragment by PCR.
Avian thymic hormone and chicken (muscle) parvalbumin are distinct proteins: isolation of a muscle parvalbumin cDNA fragment by PCR.
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DOI：
10.1016/0006-291x(91)90928-z
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发表时间：
1991-04
影响因子：
3.1
通讯作者：
W. Palmisano;M. Henzl
中科院分区：
文献类型：
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作者：
W. Palmisano;M. Henzl
通过聚合酶链式反应获得了鸡肌肉中小白蛋白的核苷酸序列。在缺乏特定氨基酸序列数据的情况下，PCR 引物基于两个小清蛋白 Ca2+ 结合位点的共有数据。通过扩增获得的 137 bp 片段清楚地编码小清蛋白，这是通过引物侧翼序列中存在 10 个不变密码子来判断的。当用于探测鸡肌肉中的 Poly(A)+RNA 时，该片段可识别 800 个核苷酸的转录物。肌肉蛋白的翻译核苷酸序列与胸腺特异性小清蛋白（称为禽类胸腺激素）的核苷酸序列明显不同。在 31 个扩增残基中，这两种蛋白质有 14 个不同。鸡胸腺中存在独特的小清蛋白，这与该蛋白质的有效效应作用一致。
Access to the nucleotide sequence of parvalbumin from chicken muscle was gained via the polymerase chain reaction. In the absence of specific amino acid sequence data, the PCR primers were based on concensus data for the two parvalbumin Ca2+-binding sites. The 137 bp fragment obtained by amplification clearly codes for a parvalbumin, as judged by the presence of 10 invariant codons within the sequence flanked by the primers. When used to probe poly(A)+RNA from chicken muscle, the fragment recognizes an 800 nucleotide transcript. The translated nucleotide sequence of the muscle protein is unmistakably distinct from that of the thymus-specific parvalbumin known as avian thymic hormone. Of the 31 amplified residues, the two proteins differ at 14. The presence of a distinct parvalbumin in chicken thymus is consistent with the potent effector role proposed for the protein.