Chemical characterization of two fractions from Sanghuangporus sanghuang and evaluation of antidiabetic activity
Chemical characterization of two fractions from Sanghuangporus sanghuang and evaluation of antidiabetic activity
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桑黄两个部位的化学表征及抗糖尿病活性评价
DOI：
10.1016/j.jff.2021.104825
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发表时间：
2021-10-28
影响因子：
5.6
通讯作者：
Tian, Xuemei
中科院分区：
文献类型：
--
作者：
Hou, Ranran;Zhou, Linjiang;Tian, Xuemei
从桑黄发酵液中提取桑黄茯苓多糖（SHP）和醇沉淀液（SHE）中的乙醇组分。气相色谱-质谱分析表明，4种有机试剂（石油醚、三氯乙烷、乙酸乙酯和正丁醇）萃取物主要为酯类、醇类、酚类、游离生物碱和苷类。在胰岛素抵抗细胞模型上测试了SHP/ SHEs的细胞毒性和降糖作用，结果表明SHP和SHEs在一定浓度下可显著促进葡萄糖消耗。有趣的是，在2.5 ~ 10 mg/mL时，SHP不仅具有降糖作用，而且具有抑制细胞活性的作用。随后，II型糖尿病模型小鼠实验结果显示，在100-400 mg/Kg.BW剂量下，SHP能显著降低血糖，恢复血脂水平，修复受损组织。综上所述，我们的研究表明，SHP具有抗糖尿病活性，作为功能食品和医疗保健品具有广阔的应用前景。
Sanghuangporus sanghuang polysaccharide (SHP) and ethyl alcohol fractions from the alcohol deposit fluid (SHE) were obtained from the fermentation broth of S. sanghuang. The component analysis by GC-MS showed that 4 organic reagent (petroleum ether, trichloroethane, ethyl acetate and n-butyl alcohol) extractions (SHEs) covered mainly esters, alcohols, phenols, free alkaloids and glycosides. The cytotoxicity and hypoglycemic effect of SHP/ SHEs were tested on insulin resistance cell model, the results showed that SHP and SHEs could significantly promote glucose consumption at certain concentrations. Interestingly, SHP not only possessed hypoglycemic, but also inhibited the cytoactive at 2.5-10 mg/mL. Subsequently, the results in type II diabetes model on mice revealed that SHP could significantly decrease blood glucose, recover blood lipid levels and repair the damaged tissues at 100-400 mg/Kg.BW. Altogether, our research concluded that SHP possesses antidiabetic activity, suggesting it a prospect application prospect as functional food and medical health product.