Intrarenal localization of interleukin-1β mRNA in crescentic glomerulonephritis
Intrarenal localization of interleukin-1β mRNA in crescentic glomerulonephritis
复制标题
新月体肾小球肾炎中白细胞介素 1β mRNA 的肾内定位
DOI：
10.1093/ndt/9.9.1228
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发表时间：
1994
影响因子：
6.1
通讯作者：
A. Cumming
中科院分区：
文献类型：
--
作者：
D. Jenkins;D. Wojtacha;P. Swan;S. Fleming;A. Cumming
IL-1 β是一种有效的促炎肽，并诱导参与免疫应答的其他细胞因子的表达。采用原位杂交技术对9例新月体肾炎患者的肾活检组织进行了研究，以确定IL-1 β mRNA的表达位点。9例非增生性肾病患者的活检作为阴性对照，扁桃体组织作为阳性对照。IL-1 β cDNA探针用随机引物32 P标记，并在脱蜡后与石蜡包埋的组织切片杂交。IL-1 β mRNA在扁桃体中表达，而在阴性对照中不表达。9例新月体活检中4例mRNA表达阳性。这在具有邻近肾小管细胞的巨噬细胞形态特征的间质细胞、肾小球簇内细胞和肾小管上皮细胞中观察到。IL-1 β mRNA在新月体肾炎肾组织中表达。肾小管和间质中IL-1 β mRNA的表达与肾小球表达同等显著。肾内细胞因子的合成可能参与新月体肾炎的发病机制。
: Il-1 beta is a potent proinflammatory peptide, and induces expression of other cytokines which are involved in the immune response. Kidney biopsies from nine patients with crescentic GN were studied by in-situ hybridization to determine the site of expression of Il-1 beta mRNA. Biopsies from nine patients with nonproliferative renal disease were studied as negative controls, and tonsillar tissue was studied as a positive control. An Il-1 beta cDNA probe was 32P-labelled by random primers and hybridized to paraffin-embedded tissue sections after de-waxing. Il-1 beta mRNA was expressed in tonsil, but not in negative controls. Positive mRNA expression was seen in four of the nine crescentic biopsies. This was observed in interstitial cells with morphological characteristics of macrophages adjacent to tubular cells, in cells within the glomerular tuft, and in tubular epithelial cells. Il-1 beta mRNA is expressed in renal tissue in crescentic GN. Tubular and interstitial expression of Il-1 beta mRNA appears of equal prominence to glomerular expression. Intrarenal cytokine synthesis may be involved in the pathogenesis of crescentic glomerulonephritis.