Real Time Monitoring of Multiple Parameters in Mammalian Cell Culture Bioreactors Using an In-Line Raman Spectroscopy Probe
Real Time Monitoring of Multiple Parameters in Mammalian Cell Culture Bioreactors Using an In-Line Raman Spectroscopy Probe
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DOI：
10.1002/bit.23023
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发表时间：
2011-05-01
影响因子：
3.8
通讯作者：
Li, Zheng Jian
中科院分区：
文献类型：
--
作者：
Abu-Absi, Nicholas R.;Kenty, Brian M.;Li, Zheng Jian
FDA的过程分析技术倡议鼓励药品制造商应用创新思想，以更好地了解他们的过程。应用这些技术来监测用于生物制剂生产的哺乳动物细胞培养生物反应器存在许多挑战。这些包括非侵入性和在线监测复杂培养基配方中多种组分的能力。我们报告的结果表明，第一次，在线应用拉曼光谱监测哺乳动物细胞培养生物反应器的技术可行性。使用拉曼探针同时预测培养参数，包括谷氨酰胺、谷氨酸、葡萄糖、乳酸、铵、活细胞密度和总细胞密度。Biotechnol. Bioeng. 2011;108：1215-1221. (C)2010 Wiley Periodicals，Inc.
The FDA's process analytical technology initiative encourages drug manufacturers to apply innovative ideas to better understand their processes. There are many challenges to applying these techniques to monitor mammalian cell culture bioreactors for biologics manufacturing. These include the ability to monitor multiple components in complex medium formulations non-invasively and in-line. We report results that demonstrate, for the first time, the technical feasibility of the in-line application of Raman spectroscopy for monitoring a mammalian cell culture bioreactor. A Raman probe was used for the simultaneous prediction of culture parameters including glutamine, glutamate, glucose, lactate, ammonium, viable cell density, and total cell density. Biotechnol. Bioeng. 2011;108: 1215-1221. (C) 2010 Wiley Periodicals, Inc.