Orcokinin-like immunoreactivity in central neurons innervating the salivary glands and hindgut of ixodid ticks.
Orcokinin-like immunoreactivity in central neurons innervating the salivary glands and hindgut of ixodid ticks.
复制标题
支配蜱蜱唾液腺和后肠的中枢神经元中的 Orcokinin 样免疫反应性。
DOI：
10.1007/s00441-015-2121-z
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发表时间：
2015
影响因子：
3.6
通讯作者：
Dušan,Žitňan
中科院分区：
文献类型：
--
作者：
Ladislav,Roller;Ladislav,Šimo;Akira,Mizoguchi;Mirko,Slovák;Yoonseong,Park;Dušan,Žitňan
Orcokinins是节肢动物门内保守的神经肽，但其在蜱中的细胞分布和功能尚不清楚。我们使用一种针对成熟的orcokinin肽的高度保守的N-末端（NFDEIDR）的抗体来检查它们在六种硬蜱中的分布：Amblyomma variegatum，Dermacentor reticulatus，Hyalomma anatolicum，Ixodes scapularis，Ixodes ricinus和Rhipicephalus approxulatus。在所有检查的蜱种属中，在突触神经节（中枢神经系统）的各个区域检测到大量免疫反应神经元（约100个）。在食管后区的两个突出群体的传出神经元的免疫反应性投射进行了详细检查：（1）神经支配的唾液腺的神经元;（2）神经支配的后肠。使用基质辅助激光解吸/电离飞行时间（MALDI-TOF），我们检测到orcokinin峰的提取物的突触和后肠，但不是在唾液腺的I。肩胛骨女性我们的数据提供了进一步的证据orcokinin在硬蜱的存在下，并确定肽能神经元网络的功能研究建立了形态学基础。
Orcokinins are conserved neuropeptides within the Arthropoda but their cellular distribution and functions in ticks are unknown. We use an antibody against the highly conserved N-terminal (NFDEIDR) of mature orcokinin peptides to examine their distribution in six ixodid species:Amblyomma variegatum,Dermacentor reticulatus,Hyalomma anatolicum,Ixodes scapularis,Ixodes ricinusandRhipicephalus appendiculatus. Numerous immunoreactive neurons (~100) were detected in various regions of the synganglion (central nervous system) in all examined tick species. Immunoreactive projections of two prominent groups of efferent neurons in the post-oesophageal region were examined in detail: (1) neurons innervating the salivary glands; (2) neurons innervating the hindgut. Using matrix-assisted laser desorption/ionisation–time-of-flight (MALDI-TOF), we detected orcokinin peaks in extracts of the synganglia and hindguts but not in the salivary glands ofI. scapularisfemales. Our data provide further evidence of the presence of orcokinin in ixodid ticks and establish a morphological basis for functional studies of identified peptidergic neuronal networks.