Genetic and chemical characterization of white and red peony root derived from Paeonia lactiflora.
Genetic and chemical characterization of white and red peony root derived from Paeonia lactiflora.
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DOI：
10.1007/s11418-014-0857-5
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发表时间：
2015-01
影响因子：
3.3
通讯作者：
Komatsu K
中科院分区：
文献类型：
--
作者：
Zhu S;Yu X;Wu Y;Shiraishi F;Kawahara N;Komatsu K
白牡丹(WPR)和赤牡丹(RPR)是中药中的两种不同的药用植物，但它们大多来源于同一植物学来源的赤牡丹。WPR和RPR之间的区别争论了很长时间。本研究试图阐明WPR和RPR的遗传和化学特性，为它们的鉴定和有效利用提供科学的数据集。对产自日本的乳香、牡丹及两个近缘种药材及药材中的核糖体DNA内转录间隔区(ITS)序列及8种主要活性成分的含量进行了分析。根据ITS序列的相似性，将产于中国南部的白粉菌和产于中国北部的白粉菌明显划分为乳花青霉两个亚群。ITS序列上游69、458和523位核苷酸可作为区分WPR和RPR的分子标记。定量分析表明，白藜芦醇苷和丹皮酚的含量明显高于中国产的南方白藜芦醇片和日本产的牡丹根粉。从中国市场上买到的牡丹皮膏通常采用硫磺熏蒸的方法进行加工，导致芍药苷的含量极低。这一研究表明，WPR和RPR不仅在地理上是分离的，而且在遗传和化学上也是分离的。ITS序列为它们的鉴定提供了遗传指标。
Two kinds of peony roots—white peony root (WPR) and red peony root (RPR)—are used for different remedies in traditional Chinese medicine; however, most of them are derived from the same botanical origin, Paeonia lactiflora. The difference between WPR and RPR has been debated for a long time. This study attempted to clarify the genetic and chemical characteristics of WPR and RPR in order to provide a scientific dataset for their identification and effective use. The nucleotide sequence of nrDNA internal transcribed spacer (ITS) and the contents of 8 main bioactive constituents were analyzed from specimens of P. lactiflora, P. veitchii and two related species as well as crude drug samples of WPR, RPR and peony root produced in Japan. Of the samples derived from P. lactiflora, the WPR produced in the southern parts of China and the RPR produced in the northern parts of China were clearly divided into two subgroups within the P. lactiflora group based on similarity of the ITS sequences. The nucleotides at positions 69, 458 and 523 upstream of the ITS sequence served as molecular markers to discriminate between WPR and RPR. Quantitative analysis indicated that the RPR samples obviously contained a higher content of paeoniflorin and paeonol, but a lower content of albiflorin than the WPR produced in the southern parts of China and peony root produced in Japan. The WPR available from Chinese markets was usually processed by sulfur fumigation, which resulted in an extremely low content of paeoniflorin. This study indicated that WPR and RPR were not only geographically isolated, but also genetically and chemically separated. The ITS sequence provided a genetic index for their identification.