Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease.
Establishment of an ELISA to detect anti-glycyl-tRNA synthetase antibody (anti-EJ), a serological marker of dermatomyositis/polymyositis and interstitial lung disease.
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DOI：
10.1016/j.cca.2014.01.005
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
H. Hane;Y. Muro;Kanako Watanabe;Y. Ogawa;K. Sugiura;M. Akiyama
中科院分区：
文献类型：
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作者：
H. Hane;Y. Muro;Kanako Watanabe;Y. Ogawa;K. Sugiura;M. Akiyama
研究背景氨酰转运RNA合成酶（aminoacyltransferRNA synthetases，ARSs）是一类在翻译过程中将氨基酸装载到同源转运RNA上的酶。先前的报告显示了针对8种不同ARS的自身抗体。虽然抗甘氨酰-tRNA合成酶抗体（抗-EJ）主要发现于炎性肌病患者，其临床意义的信息是有限的，部分原因是缺乏市售tests.MethodsWe开发了一种ELISA和免疫沉淀法，通过使用重组EJ蛋白检测抗-EJ的453例各种自身免疫性结缔组织疾病（ACTDs）。同时观察了IL-1β、IFN-γ和IFN-α 3种细胞因子对人胚肺成纤维细胞EJ mRNA和蛋白表达的影响。尽管其中3例患者患有皮肌炎/多发性肌炎，但另外2例患者没有肌炎。3例ELISA检测抗EJ抗体水平高的患者合并间质性肺病。结论利用重组EJ蛋白建立了检测抗EJ抗体的ELISA方法。这种简单易用的ELISA可以帮助澄清抗EJ在ACTD中的临床意义。
BackgroundThe aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests.MethodsWe developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines—IL-1β, IFN-γ and IFN-α—on the level of EJ mRNA and protein expressed by human fetal lung fibroblasts.ResultsFive patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines.ConclusionWe developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.