Molecular characterization of the Brassica rapa auxin-repressed, superfamily genes, BrARP1 and BrDRM1
Molecular characterization of the Brassica rapa auxin-repressed, superfamily genes, BrARP1 and BrDRM1
复制标题
DOI：
10.1007/s11033-012-2050-9
复制
发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Hur, Yoonkang
中科院分区：
文献类型：
--
作者：
Lee, Jeongyeo;Han, Ching-Tack;Hur, Yoonkang
生长素抑制超家族基因生长素抑制蛋白1 （ARP1）和休眠相关蛋白1 （DRM1）在几种植物的休眠芽和非生长组织中都有高表达。为了进一步确定这些蛋白在大白菜中的功能。我们研究了BrARP1和BrDRM1在不同发育和应激条件下的综合表达模式。我们还在转基因拟南芥植物中检测了这些相同的基因。这两种基因在所有测试的组织中都有表达，但在成熟组织中表达水平最高，并伴有低水平的生长相关标志物B. rapa核糖体蛋白27。这两个基因的表达均受到低温、热休克和盐处理等非生物胁迫的诱导。BrARP1或BrDRM1在拟南芥中的过表达会导致营养生长和种子产量降低，但不影响形态。叶柄和叶柄的长度大大缩短。这两个基因同时表达对生长抑制具有加性效应，导致植株大小显著减小。敲除拟南芥的ARP1、DRM1或两者，既不影响生长速度，也不影响最终大小。结果表明，BrARP1和BrDRM1可能通过抑制细胞伸长或细胞扩增而参与生长阻滞或停止生长，从而产生“生长制动器”。
Two auxin-repressed superfamily genes, auxin-repressed protein 1 (ARP1) and dormancy-associated protein 1 (DRM1), are highly expressed in both the dormant buds and non-growing tissues of several plant species. To further identify the function of these proteins in Chinese cabbage (Brassica rapa L. ssp. pekinensis), we examined comprehensive expression patterns of BrARP1 and BrDRM1 under various developmental and stress conditions. We also examined these same genes in transgenic Arabidopsis plants. Both genes were expressed in all tissues tested, but their levels were highest in mature tissues accompanied by low levels of the growth-associated marker, B. rapa ribosomal protein 27. Expression of both genes was induced by abiotic stresses, such as chilling, heat shock, and salt treatment. Overexpression of either BrARP1 or BrDRM1 in Arabidopsis causes a reduction in vegetative growth and seed productivity, without affecting morphology. The lengths of petioles and siliques were greatly reduced. Simultaneous expression of both genes showed an additive effect on the growth suppression, resulting in significant reduction in plant size. Knock-out of Arabidopsis ARP1, DRM1, or both, neither affected growth rate nor final size. Results suggest BrARP1 and BrDRM1 are either involved in growth arrest, or stop growth, possibly from inhibition of either cell elongation or cell expansion, thereby creating a "growth brake".