The natural and engineered 3D microenvironment as a regulatory cue during stem cell fate determination.
The natural and engineered 3D microenvironment as a regulatory cue during stem cell fate determination.
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DOI：
10.1089/ten.teb.2009.0270
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Plopper GE
中科院分区：
文献类型：
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作者：
Lund AW;Yener B;Stegemann JP;Plopper GE
使用干细胞作为再生医学中健康细胞的自我更新来源的概念已经存在了几十年，但大多数应用尚未取得临床成功。缺乏成功的干细胞疗法的主要原因是难以完全重建天然干细胞小生境的维持和控制。因此，改善移植干细胞的性能需要更好地理解在天然和工程三维微环境中指导干细胞行为的细胞机制。然而，用于揭示控制体外细胞行为的机制的大多数技术已经使用二维细胞培养物开发，并且在三维环境如工程化组织构建体中使用有限。因此，破译在天然和工程3D环境中控制干细胞命运的机制需要严格的定量技术，这些技术允许对细胞-微环境相互作用的机制、假设驱动的研究。在这里，我们回顾了目前对2D和3D干细胞控制机制的理解，并提出了一种方法来揭示3D中管理干细胞行为的机制。
The concept of using stem cells as self renewing sources of healthy cells in regenerative medicine has existed for decades, but most applications have yet to achieve clinical success. A main reason for the lack of successful stem cell therapies is the difficulty in fully recreating the maintenance and control of the native stem cell niche. Improving the performance of transplanted stem cells therefore requires a better understanding of the cellular mechanisms guiding stem cell behavior in both native and engineered three dimensional microenvironments. Most techniques, however, for uncovering mechanisms controlling cell behavior in vitro have been developed using two dimensional cell cultures and are of limited use in three dimensional environments such as engineered tissue constructs. Deciphering the mechanisms controlling stem cell fate in native and engineered 3D environments, therefore, requires rigorous quantitative techniques that permit mechanistic, hypothesis-driven studies of cell-microenvironment interactions. Here, we review the current understanding of 2D and 3D stem cell control mechanisms and propose an approach to uncovering the mechanisms that govern stem cell behavior in 3D.