Molecular Characterization of Multidrug-Resistant Pseudomonas aeruginosa Isolates in Hospitals in Myanmar
Molecular Characterization of Multidrug-Resistant Pseudomonas aeruginosa Isolates in Hospitals in Myanmar
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DOI：
10.1128/aac.02397-18
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发表时间：
2019-05-01
影响因子：
4.9
通讯作者：
Tin, Htay Htay
中科院分区：
文献类型：
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作者：
Tada, Tatsuya;Hishinuma, Tomomi;Tin, Htay Htay
多重耐药（MDR）铜绿假单胞菌的出现已成为一个严重的世界性医学问题。本研究旨在阐明从缅甸医院分离的耐多药铜绿假单胞菌菌株的遗传和流行病学特性。使用肉汤微量稀释法对从缅甸七家医院的不同患者获得的 45 株多重耐药铜绿假单胞菌分离株进行了筛选。使用 MiSeq 平台 (Illumina) 对 MDR 分离株的整个基因组进行测序。系统发育树是由单核苷酸多态性串联体构建的。推导了多位点序列类型，并鉴定了耐药基因。在45个分离株中，38个含有编码碳青霉烯酶的基因，包括DIM-1、IMP-1、NDM-1、VIM-2和VIM-5，9个分离株含有编码16S rRNA甲基化酶的基因，包括RmtB、RmtD3、RmtE和RmtF2。在缅甸分离的大多数 MDR 铜绿假单胞菌菌株属于序列类型 1047 (ST1047)。这是缅甸首次对耐多药铜绿假单胞菌临床分离株进行分子流行病学分析。这些发现强烈表明，含有碳青霉烯酶（包括 DIM-、IMP-、NDM- 和 VIM-型金属-β-内酰胺酶）的铜绿假单胞菌 ST1047 菌株已在缅甸的整个医疗环境中传播。
The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR P. aeruginosa strains isolated from hospitals in Myanmar. Forty-five MDR P. aeruginosa isolates obtained from different patients in seven hospitals in Myanmar were screened using the broth microdilution method. The whole genomes of the MDR isolates were sequenced using a MiSeq platform (Illumina). Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence types were deduced, and drug resistance genes were identified. Of the 45 isolates, 38 harbored genes encoding carbapenemases, including DIM-1, IMP-1, NDM-1, VIM-2, and VIM-5, and 9 isolates had genes encoding 16S rRNA methylases, including RmtB, RmtD3, RmtE, and RmtF2. Most MDR P. aeruginosa strains isolated in Myanmar belonged to sequence type 1047 (ST1047). This is the first molecular epidemiological analysis of MDR P. aeruginosa clinical isolates in Myanmar. These findings strongly suggest that P. aeruginosa ST1047 strains harboring carbapenemases, including DIM-, IMP-, NDM-, and VIM-type metallo-beta-lactamases, have been spreading throughout medical settings in Myanmar.