headcase, an imaginal specific gene required for adult morphogenesis in Drosophila melanogaster.
headcase, an imaginal specific gene required for adult morphogenesis in Drosophila melanogaster.
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DOI：
10.1242/dev.121.12.4149
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发表时间：
1995-12
期刊：
影响因子：
4.6
通讯作者：
Thomas A. Weaver;R. White
中科院分区：
文献类型：
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作者：
Thomas A. Weaver;R. White
像黑腹果蝇这样的全代谢昆虫的大部分成体器官都来源于特定的想象细胞。这些细胞在许多重要的细胞特征上与它们的幼虫不同，从细胞周期的性质到细胞分化的时间和模式。在这里，我们描述了一个基因的细胞，分子和遗传特征，头（hdc），这是想象细胞发育所必需的。HDC是第一个被描述的基因，在所有的想象细胞中特异性表达；这使我们能够在胚胎和幼虫发育中识别许多想象的原基。Hdc蛋白是一种极碱性（pI 9.6）的细胞质蛋白，没有明显的序列相似性或保守基序。有趣的是，hdc的时空表达模式预示着细胞重新进入有丝分裂周期，并持续到最终的细胞分裂。HDC空等位基因已被分离出来并发现可导致蛹致死，死亡的成虫在许多成虫组织的分化中表现出缺陷，最明显的是头部发育。hdc的异位表达，由hdc-minigene提供，挽救了蛹的致死率。无突变体的象盘形态表现正常，因此hdc的表达缺失并不影响象盘细胞的生长，而是干扰了蛹发育过程中象盘原基正常分化的能力，提示hdc可能参与了变态过程中的激素反应。
The majority of adult organs of a holometabolic insect like Drosophila melanogaster are derived from specific imaginal cells. These cells differ from their larval equivalents in many important cellular characteristics, ranging from the nature of the cell cycle to the timing and pattern of cellular differentiation. Here we describe the cellular, molecular and genetic characterization of a gene, headcase (hdc), which is required for imaginal cell development. hdc is the first gene to be described which is specifically expressed in all imaginal cells; this has allowed us to identify many imaginal primordia in the embryo and larval development. The Hdc protein is an extremely basic (pI 9.6) cytoplasmic protein with no obvious sequence similarities or conserved motifs. Interestingly, the spatial-temporal pattern of hdc expression prefigures imaginal cell re-entry into the mitotic cell cycle and persists until the final cell divisions. hdc null alleles have been isolated and found to cause pupal lethality, with dead pharate adults exhibiting defects in the differentiation of many adult tissues, most notably in head development. Ectopic expression of hdc, provided by a hdc-minigene, rescues the pupal lethality. Imaginal disc morphology in null mutants appears normal, therefore loss of hdc expression does not affect imaginal cell growth, but instead interferes with the ability of the imaginal primordia to differentiate properly during pupal development, suggesting that hdc may be involved in hormonal responsiveness during metamorphosis.