Light-Responsive Dynamic Protein Hydrogels Based on LOVTRAP
Light-Responsive Dynamic Protein Hydrogels Based on LOVTRAP
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DOI：
10.1021/acs.langmuir.1c01699
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发表时间：
2021-08-15
期刊：
影响因子：
3.9
通讯作者：
Li, Hongbin
中科院分区：
文献类型：
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作者：
Duan, Tianyu;Bian, Qingyuan;Li, Hongbin
基于蛋白质的水凝胶可以模拟天然细胞外基质（ECM）的许多方面，并且是有前途的生物医学材料，其在细胞增殖、药物/细胞递送和组织工程中找到各种应用。为了适应不同的任务，重要的是蛋白质基水凝胶的机械和/或生物化学性质可以通过外部刺激来调节。光作为调节刺激是有利的，因为它可以很容易地应用于所需的时空方式。光氧电压敏感结构域2（LOV 2）与其结合伙伴ZDark 1（zdk 1）之间的非共价结合称为LOVTRAP，是一种光响应性相互作用。LOVTRAP的结合亲和力在黑暗中比在蓝光照射下高得多。利用这些光响应性相互作用，本文中我们决定使用LOVTRAP作为交联机制来设计光响应性蛋白质水凝胶。使用含有LOV 2和zdk 1的多功能蛋白质构建块，我们成功地设计了一种光响应性蛋白质水凝胶，其粘弹性可以响应于光而改变：在黑暗中，水凝胶显示出更高的储能模量;在蓝光照射下，储能模量下降。由于LOVTRAP的非共价性质，工程化的LOVTRAP蛋白水凝胶显示出剪切稀化和自愈合特性，并用作优异的可注射蛋白水凝胶。我们预计，这类新的光响应蛋白质水凝胶将扩大动态蛋白质水凝胶的范围，并帮助开发其他光响应蛋白质水凝胶用于生物医学应用。
Protein-based hydrogels can mimic many aspects of native extracellular matrices (ECMs) and are promising biomedical materials that find various applications in cell proliferation, drug/cell delivery, and tissue engineering. To be adapted for different tasks, it is important that the mechanical and/or biochemical properties of protein-based hydrogels can be regulated by external stimuli. Light as a regulation stimulus is of advantage because it can be easily applied in demanded spatiotemporal manners. The noncovalent binding between the light-oxygen-voltage-sensing domain 2 (LOV2) and its binding partner ZDark1 (zdk1), named as LOVTRAP, is a light-responsive interaction. The binding affinity of LOVTRAP is much higher in dark than that under blue light irradiation. Taking advantage of these light-responsive interactions, herein we endeavored to use LOVTRAP as a crosslinking mechanism to engineer light-responsive protein hydrogels. Using LOV2-containing and zdk1-containing multifunctional protein building blocks, we successfully engineered a light-responsive protein hydrogel whose viscoelastic properties can change in response to light: in the dark, the hydrogel showed higher storage modulus; under blue light irradiation, the storage modulus decreased. Due to the noncovalent nature of the LOVTRAP, the engineered LOVTRAP protein hydrogels displayed shear-thinning and self-healing properties and served as an excellent injectable protein hydrogel. We anticipated that this new class of light-responsive protein hydrogels will broaden the scope of dynamic protein hydrogels and help develop other light-responsive protein hydrogels for biomedical applications.