Genetically Diverse Long-Lived Clonal Lineages of Phytophthora capsici from Pepper in Gansu, China
Genetically Diverse Long-Lived Clonal Lineages of Phytophthora capsici from Pepper in Gansu, China
复制标题
中国甘肃辣椒疫霉遗传多样性长寿克隆谱系
DOI：
10.1094/phyto-01-13-0016-r
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发表时间：
2013-09-01
期刊：
影响因子：
3.2
通讯作者：
Liu, Xili
中科院分区：
文献类型：
--
作者：
Hu, Jian;Pang, Zhili;Liu, Xili
辣椒疫霉对中国辣椒生产造成重大损失，我们的目的是调查甘肃省的种群结构。 2007 年至 2011 年间，在 24 个地点从辣椒中收集到了 279 个分离株。使用来自世界蔬菜中心 (AVRDC) 和新墨西哥州重组自交系 (NMRIL) 的品种对分离株（或子集）的简单序列重复 (SSR) 基因型、甲霜灵抗性、交配类型和生理种族进行了评估。 A1 和 A2 交配型从 9 个地点回收，甲霜灵抗性分离株从 3 个地点回收。 AVRDC 面板上测试的总共 104 个分离株解决了 5 个生理种族。 NMRIL 面板上测试的 42 个分离株均未引起明显感染。 127 个分离株的 SSR 基因分型揭示了 59 个独特的基因型，其中 42 个为单株，17 个具有 2 至 13 个分离株。多年来从多个地点回收了具有相同基因型的分离株，并且在许多情况下，具有不同的种族类型或甲霜灵敏感性。分离株分为三组，每组几乎都具有 A1 或 A2 交配类型。总体而言，长寿的遗传多样性克隆谱系似乎分散在甘肃各地，异交很少，并且克隆框架内存在功能重要的变异。
Phytophthora capsici causes significant loss to pepper production in China, and our objective was to investigate the population structure in Gansu province. Between 2007 and 2011, 279 isolates were collected from pepper at 24 locations. Isolates (or subsets) were assessed for simple sequence repeat (SSR) genotype, metalaxyl resistance, mating type, and physiological race using cultivars from the World Vegetable Center (AVRDC) and New Mexico recombinant inbred lines (NMRILs). The A1 and A2 mating types were recovered from nine locations and metalaxyl-resistant isolates from three locations. A total of 104 isolates tested on the AVRDC panel resolved five physiological races. None of 42 isolates tested on the NMRIL panel caused visible infection. SSR genotyping of 127 isolates revealed 59 unique genotypes, with 42 present as singletons and 17 having 2 to 13 isolates. Isolates with identical genotypes were recovered from multiple sites across multiple years and, in many cases, had different race types or metalaxyl sensitivities. Isolates clustered into three groups with each group having almost exclusively the A1 or A2 mating type. Overall it appears long-lived genetically diverse clonal lineages are dispersed across Gansu, outcrossing is rare, and functionally important variation exists within a clonal framework.