Fast electron transfer from cytochrome c(6) and plastocyanin to photosystem I of Chlamydomonas reinhardtii requires PsaF
Fast electron transfer from cytochrome c(6) and plastocyanin to photosystem I of Chlamydomonas reinhardtii requires PsaF
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DOI：
10.1021/bi970082c
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发表时间：
1997-05-27
期刊：
影响因子：
2.9
通讯作者：
Rochaix, JD
中科院分区：
文献类型：
--
作者：
Hippler, M;Drepper, F;Rochaix, JD
为了研究光系统I（PSI）中PsaF亚基的功能，采用交联技术和闪光吸收光谱法研究了莱茵衣藻（Chlamyderiareinhardtii）野生型和PsaF缺失突变体的PSI与质体蓝素或细胞色素c（6）的相互作用。我们表明，有效的电子转移从质体蓝素和细胞色素c（6）PSI依赖于PsaF。C. reinhardtii是质体蓝素和细胞色素c（6）都以一级动力学还原P700（+），半衰期为3 μ s，这在所研究光合生物中是独特的。
TO study the function of the PsaF subunit of photosystem I (PSI), the interactions between plastocyanin or cytochrome c(6) and PSI isolated from wild-type and a PsaF-deficient mutant of Chlamydomonas reinhardtii have been examined using cross-linking techniques and flash absorption spectroscopy. We show that efficient electron transfer from both plastocyanin and cytochrome c(6) to PSI depends on PsaF. A remarkable feature of the PSI complex of C. reinhardtii is that both plastocyanin and cytochrome c(6) reduce P700(+) with first-order kinetics and a half-time of 3 mu s, which is unique among photosynthetic organisms examined.