Three decades of nanopore sequencing.

Three decades of nanopore sequencing.
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DOI:
10.1038/nbt.3423
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发表时间:
2016-05-06
影响因子:
46.9
通讯作者:
Branton D
Branton D
中科院分区:
工程技术1区
文献类型:
--
作者:
Deamer D;Akeson M;Branton D

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测序中的一个长期目标是使用膜中的电压偏置纳米级孔来测量线性单链(ss)DNA或RNA分子通过该孔的通道。随着基于酶的方法的发展,使多核苷酸通过纳米孔,一个核碱基接一个核碱基,现在可以使用算法将通过孔的电流变化的测量值解码为DNA序列。在这个历史的角度来看,我们描述了纳米孔链测序的关键步骤,从25年前最早的概念化到最近的商业化和应用。
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current through the pore can now be decoded into a DNA sequence using an algorithm. In this Historical Perspective, we describe the key steps in nanopore strand-sequencing, from its earliest conceptualization more than 25 years ago to its recent commercialization and application.
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