Three decades of nanopore sequencing.
Three decades of nanopore sequencing.
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DOI:
10.1038/nbt.3423
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发表时间:
2016-05-06
影响因子:
46.9
通讯作者:
Branton D
中科院分区:
文献类型:
--
作者:
Deamer D;Akeson M;Branton D
A long-held goal in sequencing has been to use a voltage-biased nanoscale pore in a membrane to measure the passage of a linear, single-stranded (ss) DNA or RNA molecule through that pore. With the development of enzyme-based methods that ratchet polynucleotides through the nanopore, nucleobase-by-nucleobase, measurements of changes in the current through the pore can now be decoded into a DNA sequence using an algorithm. In this Historical Perspective, we describe the key steps in nanopore strand-sequencing, from its earliest conceptualization more than 25 years ago to its recent commercialization and application.
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