RNA POLYMERASE-II INTERACTS WITH THE PROMOTER REGION OF THE NONINDUCED HSP70 GENE IN DROSOPHIL-MELANOGASTER CELLS
RNA POLYMERASE-II INTERACTS WITH THE PROMOTER REGION OF THE NONINDUCED HSP70 GENE IN DROSOPHIL-MELANOGASTER CELLS
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DOI：
10.1128/mcb.6.11.3984
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发表时间：
1986-11-01
影响因子：
5.3
通讯作者：
LIS, JT
中科院分区：
文献类型：
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作者：
GILMOUR, DS;LIS, JT
通过使用蛋白DNA交联方法（D.S. Gilmour和J.T. Lis，Mol。Cell。Biol。5：2009-2018，1985），我们检查了果蝇中HSP70热休克基因上RNA聚合酶II的体内分布Melanogaster Schneider系列2个单元。在热休克诱导的细胞中，在整个基因上检测到高水平的RNA聚合酶II，而在未诱导的细胞中，RNA聚合酶II仅限于HSP70基因的5'端，主要是在核苷酸-12和 +之间-12和 +之间。 65相对于转录开始。 RNA聚合酶II的这种关联是显而易见的是，是否是通过用汞蒸气灯对冰冷细胞进行10分钟的紫外线辐射或40-.mu.s辐射细胞进行的，具有高能量Xenon闪光灯。我们假设RNA聚合酶II在诱导前可以访问该基因的启动子区域，并且该固定的RNA聚合酶II在转录激活机制中可能至关重要。
By using a protein-DNA cross-linking method (D.S. Gilmour and J.T. Lis, Mol. Cell. Biol. 5:2009-2018, 1985), we examined the in vivo distribution of RNA polymerase II on the hsp70 heat shock gene in Drosophila melanogaster Schneider line 2 cells. In heat shock-induced cells, a high level of RNA polymerase II was detected on the entire gene, while in noninduced cells, the RNA polymerase II was confined to the 5'' end of the hsp70 gene, predominantly between nucleotides -12 and +65 relative to the start of transcription. This association of RNA polymerase II was apparent whether the cross-linking was performed by a 10-min UV irradiation of chilled cells with mercury vapor lamps or by a 40-.mu.s irradiation cells with a high-energy xenon flash lamp. We hypothesize that RNA polymerase II has access to, and a high affinity for, the promoter region of this gene before induction, and this poised RNA polymerase II may be critical in the mechanism of transcription activation.