COMPARISON BETWEEN MACROSCOPIC AND MICROSCOPIC EVALUATION OF TUMOR RESPONSIVENESS USING THE SUBRENAL CAPSULE ASSAY
COMPARISON BETWEEN MACROSCOPIC AND MICROSCOPIC EVALUATION OF TUMOR RESPONSIVENESS USING THE SUBRENAL CAPSULE ASSAY
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DOI：
10.1016/0277-5379(83)90070-6
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SCHUCHHARDT, C
中科院分区：
文献类型：
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作者：
EDELSTEIN, MB;FIEBIG, HH;SCHUCHHARDT, C
采用正常小鼠肾下包膜法，对裸鼠连续传代生长的8例人原发卵巢肿瘤和3例人结肠癌、2例肺癌和5例卵巢癌进行组织学评价。评估结果表明，宏观和微观评估效果之间存在肿瘤和药物依赖相关性，环磷酰胺表现出良好的一致性，但阿霉素和顺铂在组织学分析中均表现出比宏观更强的肿瘤细胞杀伤。后两种药物显著增加白细胞浸润和纤维化，导致宏观测量不具代表性。当使用正常小鼠时，在未处理的对照中也可以证明不同数量的宿主细胞浸润。裸鼠的使用减少了宏观和微观评价之间的差异。
Using the subrenal capsule assay in normal mice, a histologic evaluation was made of 8 human primary ovarian tumors and 3 human colon, 2 lung and 5 ovarian carcinomas growing in serial passage in nude mice. The results of the evaluation indicated that there is a tumor- and drug-dependent correlation between the macroscopically and microscopically evaluated effects, with cyclophosphamide demonstrating excellent concordance but adriamycin and cisplatin both demonstrating consistently more tumor cell killing on histologic analysis than could be appreciated macroscopically. Leukocyte infiltration and fibrosis were greatly increased by the latter 2 drugs, leading to unrepresentative macroscopic measurements. Variable amounts of host cell infiltration can also be demonstrated in the untreated control when nomral mice are used. The use of nude mice decreases the discrepancy between macroscopic and microscopic evaluation.