Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus.
Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus.
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DOI：
10.1093/pcp/pcj054
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发表时间：
2006-07
影响因子：
4.9
通讯作者：
Ha Thu Mai;M. Nomura;K. Takegawa;E. Asamizu;S. Sato;Tomohiko Kato;S. Tabata;S. Tajima
中科院分区：
文献类型：
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作者：
Ha Thu Mai;M. Nomura;K. Takegawa;E. Asamizu;S. Sato;Tomohiko Kato;S. Tabata;S. Tajima
我们鉴定了一个类似 Sed5 的克隆 LjSYP32-1，它有助于百脉根结节组织的形成和植物生长。在Kazusa DNA研究所的刺参表达序列标签（EST）克隆数据库中，还检测到了另一个突触融合蛋白相关克隆（LjSYP32-2），并且这两个克隆的核苷酸和氨基酸序列非常相似。实时PCR和启动子分析表明，与LjSYP32-2相比，在植物各器官中LjSYP32-1的表达占优势。启动子分析和原位杂交表明，LjSYP32-1在幼根感染区周围的内皮层细胞层和正在发育的侧根的分生组织区域显着表达。为了探讨LjSYP32-1在根瘤和其他植物器官中的功能和生理作用，制备了表达正义或反义LjSYP32-1的日本 LjSYP32-1 稳定转化系。反义植物表现出显着迟缓的植物生长表型，表明 LjSYP32-1 在支持植物生长中发挥作用。在相同的转基因品系中，植物能够形成根瘤，但每株植物的乙炔还原活性降低了约50%。结节要小得多，一些结节通过共享内皮层而相互融合。这种不规则根瘤的发生率是野生型植物中观察到的两倍。数据表明 LjSYP32-1 有助于支持植物生长和正常根瘤组织分化。
We identified a Sed5-like clone LjSYP32-1 which contributes to nodule tissue formation and plant growth in Lotus japonicus. In the L. japonicus expressed sequence tag (EST) clone databases of Kazusa DNA Research Institute, another syntaxin-related clone (LjSYP32-2) was also detected, and the nucleotide and amino acid sequences of these two clone are very similar to each other. Real-time PCR and promoter analysis indicated that expression of LjSYP32-1 was dominant compared with LjSYP32-2 in the various plant organs. Promoter analysis and in situ hybridization revealed that LjSYP32-1 was expressed significantly in the inner cortex cell layer surrounding the infected zone of young nodules and in the meristem area of developing lateral root. To explore the function and physiological role of LjSYP32-1 in nodules and other plant organs, stable transformation lines of L. japonicus expressing either sense or antisense LjSYP32-1 were prepared. The antisense plants showed a significantly retarded plant growth phenotype, suggesting a role for LjSYP32-1 in supporting plant growth. In the same transgenic lines, the plants were capable of forming nodules, but the acetylene reduction activity was reduced by around 50% per plant. The nodules were much smaller and some nodules were fused to each other by sharing the inner cortex. The rate of occurrence of such irregular nodules was twice that observed in wild-type plants. The data suggest that LjSYP32-1 contributes to the support of plant growth and normal nodule tissue differentiation.