King cobra peptide OH-CATH30 as a potential candidate drug through clinic drug-resistant isolates.
King cobra peptide OH-CATH30 as a potential candidate drug through clinic drug-resistant isolates.
复制标题
通过临床耐药分离株将眼镜王蛇肽OH-CATH30作为潜在的候选药物。
DOI：
10.24272/j.issn.2095-8137.2018.025
复制
发表时间：
2018-03-18
影响因子：
4.9
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zhao F;Lan XQ;Du Y;Chen PY;Zhao J;Zhao F;Lee WH;Zhang Y
阳离子抗菌肽（AMPs）被认为是重要的候选治疗剂，对细菌、真菌和某些病毒具有有效的杀微生物特性。根据我们之前的研究结果，眼镜王蛇抗菌肽（OH-CATH）是一种34个氨基酸的肽，具有很强的抗菌活性和较弱的溶血活性。本研究的目的是评估OH-CATH30及其类似物D-OH-CATH30对临床分离株的体外疗效，并与常规使用的抗生素进行比较。本研究对584株临床分离株（2013-2016年）进行了检测，并根据CLSI指南采用肉汤微量稀释法测定了候选肽和抗生素的疗效。584株临床分离株中，85%对OH-CATH30及其类似物敏感。与抗生素相比，L-和D-OH-CATH30对革兰氏阳性菌的抑菌活性更高，对几乎所有革兰氏阴性菌的抑菌活性更强。对多重耐药鲍曼不动杆菌（MRAB）和耐甲氧西林金黄色葡萄球菌（MRSA）等不动杆菌的杀菌活性最高。OH-CATH30及其类似物的总体疗效高于常规使用的9种抗生素。OH-CATH30是一种很有前途的候选药物，用于治疗多种细菌感染，这些细菌感染对许多常规使用的抗菌药物具有耐药性。
Cationic antimicrobial peptides (AMPs) are considered as important candidate therapeutic agents, which exert potent microbicidal properties against bacteria, fungi and some viruses. Based on our previous findings king cobra cathelicidin (OH-CATH) is a 34-amino acid peptide that exerts strong antibacterial and weak hemolytic activity. The aim of this research is to evaluate the efficacy of both OH-CATH30 and its analog D-OH-CATH30 against clinical isolates comparing with routinely utilized antibiotics in vitro. In this study, 584 clinical isolates were tested (spanning 2013–2016) and the efficacy of the candidate peptides and antibiotics were determined by a broth microdilution method according to the CLSI guidelines. Among the 584 clinical isolates, 85% were susceptible to OH-CATH30 and its analogs. Both L- and D-OH-CATH30 showed higher efficacy against (toward) Gram-positive bacteria and stronger antibacterial activity against nearly all Gram-negative bacteria tested compare with antibiotics. The highest bactericidal activity was detected against Acinetobacter spp., including multi-drug-resistant Acinetobacter baumannii (MRAB) and methicillin-resistant Staphylococcus aureus (MRSA). The overall efficacy of OH-CATH30 and its analogs was higher than that of the 9 routinely used antibiotics. OH-CATH30 is a promising candidate drug for the treatment of a wide variety of bacterial infections which are resistant to many routinely used antimicrobial agents.
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DOI：
10.1126/science.1176667
发表时间：
2009-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fischbach MA;Walsh CT
通讯作者：
Walsh CT
九种中华臭蛙皮肤中含有极其丰富的抗菌肽
影响因子：
4.4
作者：
Yang, Xinwang;Lee, Wen-Hui;Zhang, Yun
通讯作者：
Zhang, Yun
影响因子：
4.8
作者：
Johansson, J;Gudmundsson, GH;Agerberth, B
通讯作者：
Agerberth, B
影响因子：
5.5
作者：
PARK, E;QUINN, MR;SCHULLERLEVIS, G
通讯作者：
SCHULLERLEVIS, G
影响因子：
7
作者：
Tashima, Alexandre K.;Zelanis, Andre;Serrano, Solange M. T.
通讯作者：
Serrano, Solange M. T.