DNA-RNA HYBRID DUPLEXES CONTAINING OLIGO(DA-RU) SEQUENCES ARE EXCEPTIONALLY UNSTABLE AND MAY FACILITATE TERMINATION OF TRANSCRIPTION
DNA-RNA HYBRID DUPLEXES CONTAINING OLIGO(DA-RU) SEQUENCES ARE EXCEPTIONALLY UNSTABLE AND MAY FACILITATE TERMINATION OF TRANSCRIPTION
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DOI：
10.1093/nar/8.10.2295
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
TINOCO, I
中科院分区：
文献类型：
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作者：
MARTIN, FH;TINOCO, I
含有(da：Ru)5序列的DNA-RNA杂化寡核苷酸双链DC(PA)5pG：RC(PU)5pG在室温下的稳定性比含有(Ra：dt)5序列的相应双链RC(PA)5pG：DC(PT)5pG至少低200倍。这一结果为大多数初级RNA转录本在连续的几个RU残基中终止，而不是在Ra残基中终止的发现提供了解释。它强烈支持这样的观点，即DNA-RNA杂交在转录生长点的不稳定性在转录终止中起作用。
A DNA-RNA hybrid oligonucleotide duplex, dC(pA)5pG:rC(pU)5pG, which contains a (dA:rU)5sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT)5sequence, rC(pA)5pG:dC(pT)5pG. This result provides an explanation for the finding that most primary RNA transcripts terminate in several consecutive rU residues, but not rA residues. It strongly supports the idea that instability of the DNA-RNA hybrid at the growing point of transcription plays a role in termination of transcription.