An effector CSEP087 from Erysiphe necator targets arginine decarboxylase VviADC to regulate host immunity in grapevine
An effector CSEP087 from Erysiphe necator targets arginine decarboxylase VviADC to regulate host immunity in grapevine
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DOI：
10.1016/j.scienta.2022.111205
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发表时间：
2022-09-20
影响因子：
4.3
通讯作者：
Wen, Ying-Qiang
中科院分区：
文献类型：
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作者：
Mu, Bo;Chen, Jinfu;Wen, Ying-Qiang
葡萄藤（葡萄属vinifera）遭受真菌病原体白粉病，其影响全世界的葡萄生产。白粉病分泌大量的效应物进入植物细胞，其调节先天免疫系统以促进感染的定殖。关于病原菌与植物互作的研究很多，但关于白粉病与葡萄藤互作中效应子的作用机制尚未见报道。在这项研究中，我们从葡萄藤白粉病分离株（葡萄白粉病NAFU 1）中鉴定了CSEP 087效应子，该效应子在感染期间上调，并含有一种分泌到植物细胞中的信号肽，这一点通过酵母分泌试验得到了验证。CSEP 087在葡萄藤叶片中的表达促进了白粉病的生长并抑制了活性氧（ROS）的产生。通过酵母双杂交系统筛选，CSEP 087与来自葡萄藤的精氨酸脱羧酶VviADC相互作用。VviADC是园艺植物和模式植物中的保守蛋白，特别定位于酶活性中心的识别和催化位点。VviADC在葡萄藤叶片中的表达抑制了白粉病的生长，并促进了植物中的ROS（包括过氧化氢）的产生。此外，VviADC增加与ROS清除活性相关的酶。我们的结果表明，白粉病效应子CSEP 087靶向VviADC，VviADC是过氧化氢产生和植物免疫的调节剂。这一发现推进了我们对葡萄-白粉病相互作用中效应子作用的理解。
Grapevine (Vitis vinifera) is suffering from the fungal pathogen powdery mildew, which affects grape production worldwide. Powdery mildew secretes a large number of effectors into plant cells, which modulate the innate immune system to promote colonization of the infection. Many studies have been conducted on the interactions between pathogens and plants, but the working mechanism of the effectors in the interaction between powdery mildew and grapevine has not been reported. In this study, we identified the CSEP087 effector from the grapevine powdery mildew isolate, Erysiphe necator NAFU1, which was upregulated during infection and contained a signal peptide that was secreted into the plant cells as verified by a yeast secretion assay. The expression of CSEP087 in grapevine leaves facilitated the growth of powdery mildew and suppressed the production of reactive oxygen species (ROS). CSEP087 interacted with VviADC, an arginine decarboxylase from grapevine, as screened by a yeast two-hybrid system. VviADC is a conserved protein in horticultural and model plants, particularly localized at the recognition and catalytic sites of the enzyme active center. The expression of VviADC in grapevine leaves inhibited the growth of powdery mildew and promoted ROS production, including hydrogen peroxide in the plants. In addition, VviADC increases the enzymes related to ROS scavenging activities. Our results demonstrate that the powdery mildew effector CSEP087 targeted VviADC, which is a regulator of hydrogen peroxide production and plant immunity. This finding advances our understanding of the role of effectors in the grapevine-powdery mildew interaction.