HYDRATION AND PREFERENTIAL MOLECULAR ADSORPTION ON TITANIUM INVITRO
HYDRATION AND PREFERENTIAL MOLECULAR ADSORPTION ON TITANIUM INVITRO
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DOI：
10.1016/0142-9612(92)90108-z
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发表时间：
1992-01-01
期刊：
影响因子：
14
通讯作者：
DUCHEYNE, P
中科院分区：
文献类型：
--
作者：
HEALY, KE;DUCHEYNE, P
表面敏感光谱，俄歇电子和X-射线光电子（XPS），被用来确定钛氧化物的组成，氧化物的化学计量，和吸附的表面物种的变化作为一个功能的暴露于人血清中的平衡电解质（血清/SIE）和8.0 mM乙二胺四乙酸在平衡电解质（EDTA/SIE）在37 ℃。浸泡前，氧化物接近理想的TiO 2，由两种类型的羟基覆盖：酸性OH（S）与氧双配位到钛，和碱性Ti-OH基团单配位。在长期暴露于两种溶液后，长达5000 h（约208 d），表面OH基团浓度增加，非元素P出现。PLVV俄歇跃迁和P2p谱表明，其峰位与磷酸盐参考化合物相似。吸附的磷酸盐物种被推测为Ti-H2PO4或Ti-HPO4-。XPS数据表明，脂蛋白和/或糖脂膜被吸附到暴露于血清/SIE的标本。使用静电键合概念的优先脂蛋白/糖脂吸附的分析有助于细化的钛组织界面的分层模型。显著特征是Ti金属不与生物环境直接接触，而是从本体金属、近化学计量的氧化物、Ca和P取代的水合氧化物、吸附的脂蛋白和糖脂、蛋白聚糖、胶原纤维和束到细胞的逐渐过渡。
Surface sensitive spectroscopies, Auger electron and X-ray photoelectron (XPS), were used to determine changes in titanium oxide composition, oxide stoichiometry, and adsorbed surface species as a function of exposure to human serum in a balanced electrolyte (serum/SIE) and 8.0 mM ethylenediaminetetraacetic acid in a balanced electrolyte (EDTA/SIE) at 37-degrees-C. Before immersion, the oxide was near ideal TiO2, covered by two types of hydroxyl groups: acidic OH(s) with oxygens doubly coordinated to titanium, and basic Ti-OH groups singly coordinated. After extended exposure to both solutions, up to 5000 h (ca. 208 d), the surface concentration of OH groups increased and non-elemental P appeared. The PLVV Auger transition and P 2p spectra indicated the peak positions were similar to reference phosphate compounds. The adsorbed phosphate species were presumed to be either Ti-H2PO4 or Ti-HPO4-. The XPS data suggested that a lipoprotein and/or glycolipid film was adsorbed to the specimens exposed to serum/SIE. Analysis of the preferential lipoprotein/glycolipid adsorption using electrostatic bonding concepts contributed to the refinement of the hierarchical model for the Ti-tissue interface. The salient features are that Ti metal is not in direct contact with the biological milieu, rather there is a gradual transition from the bulk metal, near-stoichiometric oxide, Ca and P substituted hydrated oxide, adsorbed lipoproteins and glycolipids, proteoglycans, collagen filaments and bundles to cells.