Complete elimination of endosymbiotic algae from Paramecium bursaria and its confirmation by diagnostic PCR
Complete elimination of endosymbiotic algae from Paramecium bursaria and its confirmation by diagnostic PCR
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草履虫中内共生藻的完全消除及其诊断性 PCR 的确认
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
H. Hosoya
中科院分区：
文献类型：
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作者：
Miho Tanaka;M. Murata;Takashi Kadono;Takashi Yamada;T. Kawano;T. Kosaka;H. Hosoya
总结。绿色草履虫，草履虫，细胞质中有数百种绿藻。共生藻类可以通过除草剂百草枯从宿主细胞中移除。通过检测藻类叶绿素的红色荧光，可以在显微镜下观察球孢子藻中共生藻类的存在。然而，荧光显微镜分析无法检测到黄化藻类。因此，应通过检测P.bursaria中是否存在藻类DNA来确认共生藻类的存在。在本研究中，我们尝试用植物基因组特异的引物扩增核酮糖-1，5-二磷酸羧基酶小亚基(RBCs)编码基因的DNA序列，用聚合酶链式反应(PCR)检测共生藻类的DNA。利用这一技术，证实了百草枯处理后的草履虫体内共生藻类的DNA缺失。
Summary. The green paramecium, Paramecium bursaria, has several hundred green algae in the cytoplasm. Symbiotic algae can be removed from their host cells by treatment with a herbicide, paraquat. The presence of symbiotic algae in P. bursaria can be microscopically examined by detecting the red fluorescence of the algal chlorophyll. However, etiolated algae could not be detected by fluorescent microscopic analyses. Therefore, the absence of symbiotic algae should be confirmed by examining the presence or absence of algal DNA in P. bursaria. In this study, we tried to detect the DNA of symbiotic algae by polymerase chain reaction (PCR) using plant genome-specific primers designed to amplify the DNA sequence in the ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) encoding gene. Using this technique, it was confirmed that the DNA of endosymbiotic algae was absent in paraquat-treated paramecia.