A novel approach employing ultrasound guidance for percutaneous cardiac muscle injection to retrograde label rat stellate ganglion neurons.
A novel approach employing ultrasound guidance for percutaneous cardiac muscle injection to retrograde label rat stellate ganglion neurons.
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一种采用超声引导经皮心肌注射逆行标记大鼠星状神经节神经元的新方法。
DOI：
10.1016/j.neulet.2004.03.087
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ruiz-Velasco,Victor
中科院分区：
文献类型：
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作者：
Fuller,BradC;Sumner,AndrewD;Kutzler,MichelleA;Ruiz-Velasco,Victor
星状神经节（SG）神经元为心脏提供主要的交感神经支配，并有助于调节心脏功能。本研究的目的是确定是否可以采用超声成像逆行标记大鼠SG神经元支配的心脏，而不采用开胸手术。此外，进行电生理实验，以表征未标记和染料标记的SG神经元中神经递质对Ca 2+通道的调制。荧光成像的实际分离的细胞显示，染料摄取是成功的5天内注射染料在心肌。全细胞电压钳记录显示，大部分的Ca 2+电流进行的N型Ca 2+通道。最后，在SG神经元暴露于去甲肾上腺素、腺苷和神经激肽A后，荧光染料摄取似乎不影响Ca 2+电流的调制。这些结果表明，超声成像引导经皮注射可以有效地用于逆行标记神经支配心脏的神经元。
Stellate ganglion (SG) neurons provide the main sympathetic innervation to the heart and help to regulate cardiac function. The purpose of this study was to determine if ultrasound imaging could be employed to retrograde label rat SG neurons innervating the heart without employing thoracotomy. In addition, electrophysiological experiments were performed to characterize the modulation of Ca2+channels by neurotransmitters in unlabeled and dye-labeled SG neurons. Fluorescence imaging of actutely isolated cells revealed that dye uptake was successful within five days following injection of dye in the cardiac muscle. Whole-cell voltage-clamp recordings revealed that the majority of the Ca2+current was carried by N-type Ca2+channels. Finally, fluorescence dye uptake did not appear to affect the modulation of Ca2+currents following exposure of SG neurons to norepinephrine, adenosine and neurokinin A. These results demonstrate that ultrasound imaging-guided percutaneous injection can be effectively employed to retrograde label neurons innervating the heart.