Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
Generation of a common marmoset embryonic stem cell line CMES40-OC harboring a POU5F1 (OCT4)-2A-mCerulean3 knock-in reporter allele
复制标题
生成含有 POU5F1 (OCT4)-2A-mCerulean3 敲入报告等位基因的常见狨猴胚胎干细胞系 CMES40-OC
DOI：
10.1016/j.scr.2021.102308
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发表时间：
2021
影响因子：
1.2
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Yoshimatsu S;Murakami R;Sato T;Saeki T;Yamamoto M;Sasaki E;Noce T;Okano H
POU 5类同源盒1（POU 5 F1，也称为OCT 4）对于维持多能性、生殖细胞命运、重编程为多能状态和早期胚胎发生至关重要。我们产生了普通绒猴（Callithrix jacchus）的胚胎干细胞（ESC）系，其具有OCT 4-P2 A-mCerulean-T2 A-pac的杂合敲入等位基因。ESC系（CMES 40-OC）将对研究致敏/幼稚多能性和生殖细胞命运有价值。同源性OCT 4敲入克隆产生，但不能在长期培养中维持在未分化状态。OCT 4敲入系统促进了在另一个基因座DDX 4（VASA）处的报告构建体的同时敲入。
POU class 5 homeobox 1(POU5F1, also known asOCT4) is critical for maintenance of pluripotency, germ cell fate, reprogramming into a pluripotent state, and early embryogenesis. We generated an embryonic stem cell (ESC) line of the common marmoset (Callithrix jacchus) harboring a heterozygous knock-in allele of OCT4-P2A-mCerulean-T2A-pac. The ESC line (CMES40-OC) will be valuable for investigation of primed/naïve pluripotency and germ cell fate. HomozygousOCT4knock-in clones were generated but could not be sustained in an undifferentiated state in long-term culture. The OCT4 knock-in system facilitated simultaneous knock-in of a reporter construct at another locus,DDX4(VASA).