A Comparative Analysis of the Venom Gland Transcriptomes of the Fishing Spiders Dolomedes mizhoanus and Dolomedes sulfurous.
A Comparative Analysis of the Venom Gland Transcriptomes of the Fishing Spiders Dolomedes mizhoanus and Dolomedes sulfurous.
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钓鱼蜘蛛Dolomedes mizhoanus和Dolomedessulfous毒液腺转录组的比较分析
DOI：
10.1371/journal.pone.0139908
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Xu X;Wang H;Zhang F;Hu Z;Liang S;Liu Z
多列目昆虫（Dolomedessulfurous）和多列目昆虫（Dolomedesmizhoanus）是捕食性节肢动物，以小鱼为食。他们生活在同一个地方，有着相似的生活习惯。它们的毒液在生物化学和电生理特性方面表现出一定的相似性和差异性。本研究首先构建了中华绒螯蟹毒腺cDNA文库，进行了转录组学分析。鉴定了127个新的推测毒素序列，将其分为8个家族。然后将该毒腺转录组与D. mizhoanus，这表明两种蜘蛛毒液的推定毒素可能起源于相同的基因祖先，尽管新毒素在两种蜘蛛中独立进化。两种蜘蛛的推定毒素含有6-12个半胱氨酸残基，形成7种半胱氨酸模式。通过blast搜索发现，这两种毒液中含有丰富的靶向离子通道的神经毒素，具有药理和治疗意义。这项研究提供了深入了解两个密切相关的蜘蛛物种的毒液，这将是用于未来的调查，其毒素的结构和功能。
Dolomedes sulfurous and Dolomedes mizhoanus are predaceous arthropods catching and feeding on small fish. They live in the same area and have similar habits. Their venoms exhibit some similarities and differences in biochemical and electrophysiological properties. In the present work, we first performed a transcriptomic analysis by constructing the venom gland cDNA library of D. sulfurous and 127 novel putative toxin sequences were consequently identified, which were classified into eight families. This venom gland transcriptome was then compared with that of D. mizhoanus, which revealed that the putative toxins from both spider venoms might have originated from the same gene ancestors although novel toxins were evolved independently in the two spiders. The putative toxins from both spiders contain 6–12 cysteine residues forming seven cysteine patterns. As revealed by blast search, the two venoms are rich in neurotoxins targeting ion channels with pharmacological and therapeutic significance. This study provides insight into the venoms of two closely related species of spider, which will be of use for future investigations into the structure and function of their toxins.
影响因子：
3
作者：
Kumar CG;LeDuc R;Gong G;Roinishivili L;Lewin HA;Liu L
通讯作者：
Liu L