Continuous hydrogen production by the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1
Continuous hydrogen production by the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1
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DOI：
10.1016/j.jbiotec.2004.11.002
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发表时间：
2005-03-30
影响因子：
4.1
通讯作者：
Imanaka, T
中科院分区：
文献类型：
--
作者：
Kanai, T;Imanaka, H;Imanaka, T
在85 ℃下对极端嗜热古菌Thermococcus kodakaraensis KOD 1的产氢潜力进行了评价。在使用补充有元素硫（S-0）的复合培养基的分批培养中，在气相中观察到H2S和CO2的释放。当省略SO并在培养基中加入丙酮酸盐或淀粉时，细胞产生高水平的H-2而不是H2S。由于H-2的水平似乎与比生长速率相关，因此在连续培养物中进行分析以开发连续H-2生产系统。在稀释率为0.2 h（-1）的稳态条件下，在补充丙酮酸盐和淀粉的培养基中观察到的连续H-2产生速率（每克干重，gdw）分别为24.9和14.0 mmol gdw（-1）h（-1）。在这两种培养中，乙酸和丙氨酸的高积累被发现作为代谢产物。当在丙酮酸盐培养基中提高稀释率时，观察到稳态生长长达0.8 h（-1），并且获得最大H-2产生速率为59.6 mmol gdw（-1）h（-1）。根据实验结果沿着的全基因组序列数据，讨论了该菌株降解淀粉和丙酮酸的代谢途径。(C)2004 Elsevier B. V.保留所有权利。
The hydrogen (H-2) production potential of the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1 was evaluated at 85 degreesC. In batch cultivation using a complex medium supplemented with elemental sulfur (S-0), evolution of H2S and CO2 was observed in the gas phase. When So was omitted and pyruvate or starch was added in the medium, the cells produced H-2 at high levels instead of H2S. As the level of H-2 appeared to correlate with the specific growth rate, analysis in continuous cultures was performed to develop a continuous H-2 production system. In a steady-state condition at a dilution rate of 0.2 h(-1), a continuous H-2 production rate (per gram dry weight, gdw) of 24.9 and 14.0 mmol gdw(-1) h(-1) was observed in media supplemented with pyruvate and starch, respectively. In both cultivations, a high accumulation of acetate and alanine was found as metabolites. When the dilution rates were elevated in the medium with pyruvate, steady-state growth was observed up to 0.8 h(-1), and a maximum H-2 production rate of 59.6 mmol gdw(-1) h(-1) was obtained. Based on the experimental results along with data of the entire genome sequence, the metabolic pathway of the strain relating to starch and pyruvate degradation is discussed. (C) 2004 Elsevier B.V. All rights reserved.