Heterologous expression of the halophyte Zoysia matrella H+-pyrophosphatase gene improved salt tolerance in Arabidopsis thaliana
Heterologous expression of the halophyte Zoysia matrella H+-pyrophosphatase gene improved salt tolerance in Arabidopsis thaliana
复制标题
盐生植物结缕草H-焦磷酸酶基因的异源表达提高了拟南芥的耐盐性
DOI：
10.1016/j.plaphy.2015.04.004
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发表时间：
2015-06-01
影响因子：
6.5
通讯作者：
Liu, Jianxiu
中科院分区：
文献类型：
--
作者：
Chen, Yu;Li, Lanlan;Liu, Jianxiu
液泡H+-焦磷酸酶（VP）家族基因在盐胁迫条件下的植物生长中起重要作用。尽管VP在植物盐胁迫调节中具有重要的生物学意义，但在盐生草坪草结缕草中尚无关于VP的报道。本研究利用退化PCR和RACE PCR方法从结缕草中分离到一个编码768个氨基酸的I型VP同源基因ZmVP1。ZmVP1的表达水平受盐度、干旱和寒冷的影响显著，但不受高温的影响。ZmVP1可以恢复盐敏感酵母菌的耐盐能力。ZmVP1在拟南芥中过表达，使拟南芥在盐胁迫下生长更加旺盛。在盐胁迫下，转基因拟南芥叶片中Na+和K+的积累量高于野生型植物，V-ATPase和V-PPase活性较高，5种胁迫相关基因（AtNHX1、AtLEA、AtP5CS、AtMn-SOD、AtAPX1）的相对表达量也较高。这些结果表明，ZmVP1是一种功能性的张力质体H+-焦磷酸酶，可能通过调节液泡内Na+区室、K+同化、渗透调节和抗氧化反应来促进耐盐性。(C) 2015 Elsevier Masson SAS。版权所有。
A number of vacuolar H+-pyrophosphatase (VP) family genes play important roles in plant growth under salt stress condition. Despite their biological importance in plant salt-stress regulation, there is no report about VP in the halophytic turfgrass Zoysia matrella. Here, we isolated ZmVP1, a type I VP homologues gene encoding 768 amino acids by using the degenerated PCR and RACE PCR methods from Zoysia matrella. The expression level of ZmVP1 was significantly induced by salinity, drought and cold, but not by heat. ZmVP1 can restore the salt-tolerant ability of a salt-sensitive yeast strain. Overexpression of ZmVP1 in Arabidopsis thaliana resulted in more vigorous growth under salt stress. Moreover, the transgenic Arabidopsis accumulated more Na+ and K+ in the leaves compared to that of wild type plants under salt stress, had higher activities of V-ATPase and V-PPase, and showed higher relative gene expression levels of 5 stress-related genes (AtNHX1, AtLEA, AtP5CS, AtMn-SOD, AtAPX1). These results demonstrated that ZmVP1 from Z matrella was a functional tonoplast H+-pyrophosphatase contributing to salt tolerance potentially through regulating the Na+ compartment in vacuole, K+ assimilation, osmotic regulation and antioxidant response. (C) 2015 Elsevier Masson SAS. All rights reserved.