Fungi and inflammatory bowel diseases:: Alterations of composition and diversity
Fungi and inflammatory bowel diseases:: Alterations of composition and diversity
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DOI：
10.1080/00365520801935434
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发表时间：
2008-01-01
影响因子：
1.9
通讯作者：
Schreiber, Stefan
中科院分区：
文献类型：
--
作者：
Ott, Stephan J.;Kuehbacher, Tanja;Schreiber, Stefan
目标。肠道黏膜相关微生物群的细菌多样性改变可能反映了与慢性炎症性肠病（IBD）发展相关的生活方式因素的净影响。虽然有报道称IBD中细菌多样性减少，但对真菌微生物群知之甚少。本研究的目的是对IBD患者肠道真菌菌群进行系统分析。材料和方法。采用基于18S rdna的宏基因组变性梯度凝胶电泳（DGGE）、克隆文库、测序和原位杂交技术，对47例对照和57例IBD患者的104个结肠活检组织的粘膜相关真菌微生物群进行了研究。结果。在所有104例患者中均可检测到真菌特异性18S rDNA特征，仅占肠道微生物群的一小部分（0.02%的粘膜和0.03%的粪便微生物群）。在克隆文库中发现了43个不同的操作分类单元（OTUs）。IBD患者与对照组真菌菌群的定性组成存在差异。DGGE谱显示，与对照组相比，克罗恩病（CD）患者的平均真菌多样性更高(CD为10.8 +/- 3.1比6.2 +/- 2.4,p
Objective. Altered bacterial diversity of the intestinal mucosa-associated microbiota may reflect the net influence of lifestyle factors associated with the development of chronic inflammatory bowel diseases (IBD). While a reduced bacterial diversity has been reported in IBD, little is known about the fungal microbiota. The aim of this study was to carry out a systematic analysis of intestinal fungal microbiota in IBD. Material and methods. The mucosa-associated fungal microbiota of 104 colonic biopsy tissues from 47 controls and 57 IBD patients was investigated using metagenomic 18S rDNA-based denaturing gradient gel electrophoresis (DGGE), clone libraries, sequencing, and in situ hybridization techniques. Results. Fungi-specific 18S rDNA signatures could be detected in all 104 patients, accounting for only a small proportion of the intestinal microbiota (0.02% of the mucosal and 0.03% of the fecal microbiota). An overall fungal biodiversity of 43 different operational taxonomic units (OTUs) was found in the clone libraries. The qualitative composition of fungal microbiota was different between patients with IBD and controls. The DGGE profiles showed a higher mean fungal diversity in patients with Crohn's disease (CD) in comparison with controls (10.8 +/- 3.1 versus 6.2 +/- 2.4 for CD, p